PXD031371

PXD031371 is an original dataset announced via ProteomeXchange.

Title	NanoTACs: NanoLuc-Targeting Protein Degraders that Parallel Existing Technology
Description	NanoLuc-targeting PROTACs (NanoTACs) hijack the CRBN and VHL cullin-RING ligase complexes to trigger proteasomal degradation of substrates tagged with the 19 kDa luminescent NanoLuc tag. In order to validate the system and confirm selective degradation of the target protein and assess any potential off-target degradation, here DDA-PASEF label-free quantification was performed to monitor target-induced degradation following addition of NC4.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_07:05:19.928.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Cobbold
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-01 01:24:23	ID requested
⏵ 1	2022-05-20 07:05:20	announced

Grohmann C, Magtoto CM, Walker JR, Chua NK, Gabrielyan A, Hall M, Cobbold SA, Mieruszynski S, Brzozowski M, Simpson DS, Dong H, Dorizzi B, Jacobsen AV, Morrish E, Silke N, Murphy JM, Heath JK, Testa A, Maniaci C, Ciulli A, Lessene G, Silke J, Feltham R, Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms. Nat Commun, 13(1):2073(2022) [pubmed]

submitter keyword: LC-MS, Protac, human

Simon Cobbold
contact affiliation	Ubiquitin Signalling Division, WEHI
contact email	cobbold.s@wehi.edu.au
lab head
Simon Cobbold
contact affiliation	WEHI
contact email	cobbold.s@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031371

PRIDE project URI

• PRIDE
  1. PXD031371
     1. Label: PRIDE project
     2. Name: NanoTACs: NanoLuc-Targeting Protein Degraders that Parallel Existing Technology