PXD031347

PXD031347 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analyses reveal the mechanical-stress-dependent changes in trabecular meshwork cytoskeletal-extracellular matrix interactions
Description	Trabecular meshwork (TM) tissue is subjected to constant mechanical stress due to the ocular pulse created by the cardiac cycle. This induces alterations in membrane lipids and associated cell-cell adhesion and cell-extracellular matrix (ECM) interactions triggering intracellular signaling responses to counter mechanical insults. A loss of such response can lead to elevated intraocular pressure (IOP), a major risk factor for primary open-angle glaucoma. The purpose of this study was to understand the response of TM under mechanical stretch using a multi-omics approach. We performed an unbiased mRNA sequencing for changes in transcripts, mass spectrometry (MS)-based quantitative proteomics for protein changes, and multiple reaction monitoring (MRM) profiling based MS and HPLC-based MS to identify lipid changes. With the help of the multi-omics data analysis on human TM cells under mechanical stress, we provide evidence for the regulation of TM actin cytoskeleton and ECM interactions at the mRNA and protein levels, which can further modulate the TM lipids contents effectively regulating the mechanical properties of TM membrane. Overall, in this study, we propose the mechanistic interplay of macromolecules to bring about a concerted cellular response in TM cells to achieve mechanotransduction and IOP regulation
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:20.692.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031347
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-31 00:55:51	ID requested
1	2022-08-30 10:44:38	announced
⏵ 2	2023-11-14 08:55:21	announced	2023-11-14: Updated project metadata.

10.6019/PXD031347;

submitter keyword: cyclic mechanical stretch,Human Trabecular meshwork, global proteomic analysis (LC-MS/MS), mRNA sequencing., lipidomic analysis

Padmanabhan Paranji Pattabiraman, PhD
contact affiliation	Assistant Professor, Department of Ophthalmology, School of Medicine, Indiana University Indianapolis, IN, USA
contact email	ppattabi@iu.edu
lab head
Emma Doud
contact affiliation	Indiana University School of Medicine
contact email	edoud@iu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031347
     1. Label: PRIDE project
     2. Name: Multi-omics analyses reveal the mechanical-stress-dependent changes in trabecular meshwork cytoskeletal-extracellular matrix interactions