PXD031342

PXD031342 is an original dataset announced via ProteomeXchange.

Title	The structural dynamics of full length divisome transmembrane proteins FtsQ, FtsB, and FtsL in FtsQBL complex formation
Description	Bacterial divisome is a group of proteins governing cell division. Out of the 15 critical proteins in Escherichia coli, the transmembrane protein complex FtsQBL plays an essential role in regulating the action. Although extensive efforts have been made, there is limited information regarding the interaction within this three-member complex. Here, HDX-MS was used to study the dynamic change of the full-length protein FtsQ, FtsB, and FtsL. Direct experimental evidence shows that FtsB and FtsL form a stable complex by the transmembrane and periplasmic regions. After the complex formation, the structural feature in FtsB properly becomes more defined. This transition may also bring the constriction control domains (CCDs) close to the one in FtsL. Furthermore, with the helical interaction between two proteins, the CCDs have a more determined location relative to the membrane surface. On the other hand, the FtsQ in the FtsQBL complex showed rigidity enhancement in two regions. The interaction with FtsB would fix a significant portion of the FtsQ β-domains. Interestingly, the formation of the FtsQBL complex also stiffens the sequence connecting the two domains in FtsQ. Overall, this study provides important experimental evidence on the conformational dynamic change of the FtsQ, FtsB, and FtsL upon the complex formation and insights into the FtsQBL function in the divisome.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:23:43.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wai Po Kong
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-30 14:31:46	ID requested
1	2022-10-13 22:38:10	announced
⏵ 2	2023-11-14 08:23:44	announced	2023-11-14: Updated project metadata.

Kong WP, Gong F, So PK, Chen YW, Chan PH, Leung YC, Wong KY, The structural dynamics of full-length divisome transmembrane proteins FtsQ, FtsB, and FtsL in FtsQBL complex formation. J Biol Chem, 298(8):102235(2022) [pubmed]

submitter keyword: FtsL, FtsQ, FtsB, hydrogen/deuterium exchange mass spectrometry,Ddivisome, HDX-MS, membrane protein

Kwok-yin Wong
contact affiliation	Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University
contact email	kwok-yin.wong@polyu.edu.hk
lab head
Wai Po Kong
contact affiliation	The Hong Kong Polytechnic University
contact email	wai-po-ball.kong@connect.polyu.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031342
     1. Label: PRIDE project
     2. Name: The structural dynamics of full length divisome transmembrane proteins FtsQ, FtsB, and FtsL in FtsQBL complex formation