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PXD031329

PXD031329 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of factors secreted by testicular germ cell tumor cell lines and microenvironmental cells.
DescriptionType II germ cell tumors (GCTs) are the most common neoplasia in young men of age 14-45 years. It is generally accepted that GCTs arise from a common precursor lesion, called germ cell neoplasia in situ (GCNIS), eventually developing into seminomas or non-seminomas, such as (embryonal carcinomas, ECs). The latter stem-cell like EC can further differentiate into teratomas (TE), yolk-sac tumors (YST), or choriocarcinomas (CC). Even though cisplatin-based chemotherapy is an efficacious standard of care during the management of GCT patients, the development of cisplatin resistance remains a major obstacle. As such, the surrounding tumor microenvironment and its secreted factors could endorse the development of drug resistance. These stromal cells, including immune cells (e.g. macrophages, T-lymphocytes), endothelial cells, and fibroblasts, can influence tumor cells by promoting proliferative and anti-apoptotic signaling pathways. Vice versa, microenvironmental cells can be influenced by tumor cells as well. For the identification of secreted proteins, cell lysates and supernatants from seven human germ cell tumor cell lines (seminoma: TCam-2; embryonal carcinoma (EC): 2102EP, NCCIT; choriocarcinoma (CC): JAR, JEG-3), yolk-sac tumor (YST): GCT72; 1411H) and five human cell types from the microenvironment (fibroblasts: MPAF, HVHF2; M2 macrophages: THP-1-M2; T-lymphocytes: JURKAT; endothelial cells: HUVEC) have been evaluated using liquid chromatography coupled with mass spectrometry (LC-MS).
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:34:35.506.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGereon Poschmann
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-30 10:57:33ID requested
12022-10-13 05:33:52announced
22023-11-14 07:34:37announced2023-11-14: Updated project metadata.
Publication List
Skowron MA, Eul K, Stephan A, Ludwig GF, Wakileh GA, Bister A, S, ö, hngen C, Raba K, Petzsch P, Poschmann G, Kuffour EO, Degrandi D, Ali S, Wiek C, Hanenberg H, M, ü, nk C, St, ü, hler K, K, ö, hrer K, Mass E, Nettersheim D, Profiling the 3D interaction between germ cell tumors and microenvironmental cells at the transcriptome and secretome level. Mol Oncol, 16(17):3107-3127(2022) [pubmed]
Keyword List
submitter keyword: germ cell tumors, liquid chromatography coupled mass spectrometry, tumor microenvironment, secretome
Contact List
Gereon Poschmann
contact affiliationProteome Research (AG Stühler) Institute of Molecular Medicine Heinrich Heine University Düsseldorf 40225 Düsseldorf Germany
contact emailgereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliationMolecular Proteomics Laboratory
contact emailgereon.poschmann@hhu.de
dataset submitter
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Dataset FTP location
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