PXD031293

PXD031293 is an original dataset announced via ProteomeXchange.

Title	Suppression of cytochrome c oxidase subunit 4 isoform 1 expression inhibits replication of highly pathogenic H5N1 influenza virus
Description	Human infection with highly pathogenic H5N1 influenza virus causes severe respiratory diseases. Currently, the drugs against H5N1 are limited to virus-targeted inhibitors. However, drug resistance caused by these inhibitors is becoming a serious threat to global public health. An alternative strategy to reduce the resistance risk is to develop antiviral drugs targeting host cell proteins. In this study, we demonstrated that cytochrome c oxidase subunit 4 isoform 1 (COX41) of host cell plays an important role in H5N1 infection. Overexpression of COX41 promoted the viral replication, which was inhibited by silencing or knock-out the expression of COX41 in host cell. The viral ribonucleoproteins (vRNP) were retained in the nucleus after down-regulation of cellular COX41 expression. Strikingly, suppressing the expression of COX41 by lycorine, a small-molecule inhibitor isolated from Amaryllidaceae plants, resulted in the blockage of nuclear export of vRNP and inhibition of viral replication both in vitro and in vivo. Importantly, we did not obtain the resistant virus after culturing the virus with the continuous treatment of lycorine. Collectively, these results provided the direct evidence of the positive correlation between cellular COX41 levels and the influenza virus infection. Inhibition of COX41 expression in host cell may serve as a viable approach for anti-influenza therapy
HostingRepository	iProX
AnnounceDate	2022-01-27
AnnouncementXML	Submission_2022-10-09_00:14:09.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun He
SpeciesList	scientific name: Canis lupus; NCBI TaxID: 9612;
ModificationList	isotope labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-26 20:25:01	ID requested
⏵ 1	2022-10-09 00:14:10	announced

He J, Huang H, Li B, Li H, Zhao Y, Li Y, Ye W, Qi W, Tang W, Wang L, Identification of cytochrome c oxidase subunit 4 isoform 1 as a positive regulator of influenza virus replication. Front Microbiol, 13():862205(2022) [pubmed]

submitter keyword: Highly pathogenic H5N1 influenza virus, Lycorine, Anti-influenza, Viral ribonucleoproteins export, COX41, Amaryllidaceae plants

Lei Wang
contact affiliation	Centre for Bioactive Natural Molecules and Innovative Drugs, and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University
contact email	cpuwanglei@126.com
lab head
Jun He
contact affiliation	Centre for Bioactive Natural Molecules and Innovative Drugs, and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University
contact email	hejun@jnu.edu.cn
dataset submitter

iProX dataset URI