PXD031266

PXD031266 is an original dataset announced via ProteomeXchange.

Title	Isolation and determination of PI(4,5)P2 proximal proteins
Description	We screened proteins in close proximity to PI(4,5)P2 in epithelial cells. We established HaCaT cells stably expressing APEX2 N-terminally fused to the PH domain of PLCd1, which specifically binds to PI(4,5)P2. In the presence of hydrogen peroxide and biotin-phenol, proteins proximal to APEX2 are biotinylated by APEX2, allowing for their enrichment using streptavidin beads. APEX2-fused non-PI(4,5) P2-binding mutant of the PH domain of PLCd1 (R40L) was used as the negative control.
HostingRepository	PRIDE
AnnounceDate	2022-03-26
AnnouncementXML	Submission_2022-03-26_01:45:25.434.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031266
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yoshikazu Nakamura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-26 06:35:06	ID requested
⏵ 1	2022-03-26 01:45:25	announced

Dataset with its publication pending

submitter keyword: SILAC PI(4,5)P2 PLCd1 PH

Yoshikazu nakamura
contact affiliation	Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan.
contact email	ynakamur@rs.tus.ac.jp
lab head
Yoshikazu Nakamura
contact affiliation	Tokyo University of Science
contact email	ynakamur@rs.tus.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031266
     1. Label: PRIDE project
     2. Name: Isolation and determination of PI(4,5)P2 proximal proteins