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PXD031261

PXD031261 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIsoleucyl-tRNA synthetase interactome analysis
DescriptionAminoacyl-tRNA synthetases (aaRSs) catalyze the ligation of each amino acid to the 3’ hydroxyl group of the cognate tRNA and thereby establish the genetic code for protein synthesis. AaRSs form a complicated network through assembly into a multi-synthetase complex (MSC), which is comprised of nine aaRSs (ArgRS, AspRS, GlnRS, GluProRS, IleRS, LeuRS, LysRS, and MetRS) and three auxiliary proteins (aaRS-interacting multifunctional proteins, p43, p38, and p18 or AIMP1, 2, and 3) in vertebrates. IleRS is one of the least characterized aaRSs in the MSC. It is a class 1a aaRS and has an UNE-I domain at its C-terminal end that is formed by tandem ~90 residue repeats. Systematic depletion and yeast two-hybrid (Y2H) analyses suggest that IleRS binds to the WHEP domain of GluProRS through its UNE-I domain. However, crosslinking and mass spectrometry (XL-MS) analyses revealed that IleRS interacts with ArgRS, LeuRS, and MetRS in the MSC. While such difference may be due to the different dynamic states of the MSC under various cellular environments, it remains to be resolved how IleRS associates with other components of the MSC. Therefore, we performed mass spectrometry analysis to study IleRS interactome using cells expressing wild-type IleRS, C-terminal-deleted IleRS, and the IleRS UNE-I domain alone.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:40:18.053.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD031261
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterByung-gyu kim
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-26 02:28:36ID requested
12023-03-11 01:59:10announced
22023-11-14 08:40:18announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD031261;
Chung S, Kang MS, Alimbetov DS, Mun GI, Yunn NO, Kim Y, Kim BG, Wie M, Lee EA, Ra JS, Oh JM, Lee D, Lee K, Kim J, Han SH, Kim KT, Chung WK, Nam KH, Park J, Lee B, Kim S, Zhao W, Ryu SH, Lee YS, Myung K, Cho Y, Regulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase 1. Nat Commun, 13(1):6732(2022) [pubmed]
Keyword List
submitter keyword: tRNA synthetase, interactome,structure, LC-MS/MS
Contact List
Yunje Cho
contact affiliationDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, South Korea
contact emailyunje@postech.ac.kr
lab head
Byung-gyu kim
contact affiliationCenter for genomic integrity (CGI), Institute of basic science (IBS), UNIST, Ulsan, 44919, Republic of Korea
contact emailgoldenlion@ibs.re.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
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