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PXD031243

PXD031243 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFrom incision to excision: active DNA damage eviction by HLTF stimulates repair
DescriptionNucleotide excision repair (NER) counteracts the onset of cancer and aging by removing helix-distorting DNA lesions via a ‘cut-and-patch’-type reaction. The regulatory mechanisms that drive NER through its successive damage recognition, verification, incision and gap restoration reaction steps remain elusive. Here we show that the RAD5-related translocase HLTF facilitates repair through active eviction of incised damaged DNA together with associated repair proteins. Our data shows a dual incision-dependent recruitment of HLTF to the NER incision complex, mediated by HLTF’s HIRAN domain that binds 3’-OH single-stranded DNA ends. HLTF’s translocase motor subsequently promotes dissociation of incised oligonucleotides together with members of the stably damage-bound incision complex to allow efficient PCNA loading and initiation of repair synthesis. Our findings uncover HLTF as an important NER factor that actively evicts DNA damage, thereby providing an additional quality control by coordinating the transition between the excision and DNA synthesis steps to safeguard genome integrity.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:46:57.206.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeroen Demmers
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-25 07:32:32ID requested
12023-05-10 13:43:23announced
22023-11-14 08:46:58announced2023-11-14: Updated project metadata.
Publication List
van Toorn M, Turkyilmaz Y, Han S, Zhou D, Kim HS, Salas-Armenteros I, Kim M, Akita M, Wienholz F, Raams A, Ryu E, Kang S, Theil AF, Bezstarosti K, Tresini M, Giglia-Mari G, Demmers JA, Sch, ä, rer OD, Choi JH, Vermeulen W, Marteijn JA, Active DNA damage eviction by HLTF stimulates nucleotide excision repair. Mol Cell, 82(7):1343-1358.e8(2022) [pubmed]
Keyword List
submitter keyword: UV damage response,HLTF, repair synthesis, damage eviction, DNA damage, TFIIH, genome stability, post-replication repair, Nucleotide Excision Repair
Contact List
Jeroen Demmers
contact affiliationProteomics Center, Erasmus University Medical Center, Rotterdam - NL
contact emailj.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliationProteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact emailj.demmers@erasmusmc.nl
dataset submitter
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Dataset FTP location
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