PXD031238

PXD031238 is an original dataset announced via ProteomeXchange.

Title	Accurate proteome-wide measurement of methionine oxidation in aging mouse brains
Description	The oxidation of methionine side chains has emerged as an important posttranslational modification of proteins. A diverse array of low-throughput and targeted studies have suggested that the oxidation of methionine residues in select proteins can have diverse impacts on cell physiology, ranging from detrimental effects on protein structure and stability to functional roles in cell signaling. Despite its importance, the large-scale investigation of methionine oxidation in a complex matrix, such as the cellular proteome has been historically hampered by technical limitations. Herein we report a methodology, Methionine Oxidation by Blocking (MobB), that allows for accurate and precise quantification of low levels of methionine oxidation typically observed in vivo. To demonstrate the utility of this methodology, we applied MobB to the brain tissues of young (6 m.o.) and old (20 m.o.) mice and identified over 280 novel sites for in vivo methionine oxidation. We further demonstrated that oxidation stoichiometries for specific methionine residues are highly consistent between individual animals and methionine sulfoxides are enriched in clusters of functionally related gene products including membrane and extracellular proteins. However, we did not detect significant changes in methionine oxidation in brains of old mice, suggesting that oxidation levels are highly regulated during the course of aging.
HostingRepository	PRIDE
AnnounceDate	2022-05-23
AnnouncementXML	Submission_2022-05-23_12:55:15.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Bettinger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-25 04:07:29	ID requested
⏵ 1	2022-05-23 12:55:15	announced

Bettinger JQ, Simon M, Korotkov A, Welle KA, Hryhorenko JR, Seluanov A, Gorbunova V, Ghaemmaghami S, Accurate Proteomewide Measurement of Methionine Oxidation in Aging Mouse Brains. J Proteome Res, 21(6):1495-1509(2022) [pubmed]

submitter keyword: methionine oxidation, brain, mouse, aging

Sina Ghaemmaghami
contact affiliation	University of Rochester Department of Biology
contact email	sina.ghaemmaghami@rochester.edu
lab head
John Bettinger
contact affiliation	University of Rochester
contact email	jbetting@ur.rochester.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031238

PRIDE project URI

• PRIDE
  1. PXD031238
     1. Label: PRIDE project
     2. Name: Accurate proteome-wide measurement of methionine oxidation in aging mouse brains