PXD031228

PXD031228 is an original dataset announced via ProteomeXchange.

Title	Vascular bed systematic profiling unravels endothelial and glycocalyx dysregulations in experimental high salt hypertension
Description	Objective: Considerable evidence links dietary salt intake with the development of hypertension, left ventricular hypertrophy, and increased risk of stroke and coronary heart disease. Despite extensive epidemiological and basic science interrogation of the relationship between high salt (HS) intake and blood pressure, it remains unclear how HS impacts endothelial cell (EC) and vascular structure in vivo. This study aims to uncover the HS-induced vascular pathologies using a differential systemic decellularization in vivo (DISDIVO) approach. Approach and Results: We performed systematic molecular characterization of the endothelial glycocalyx (eGC) and EC proteomes in mice with HS (8%) diet-induced hypertension versus healthy control animals. Isolation of eGC and ECs compartments was achieved using the DISDIVO approach. Altered protein expression in hypertensive compared to normal mice was characterized by liquid chromatography tandem-mass spectrometry (LC-MS/MS). Proteomic results from eGC fractions revealed a significant downregulation of eGC and associated proteins in HS diet-induced hypertensive mice. Among 1696 proteins identified in this group, 723 were markedly downregulated while 168 were upregulated, bioinformatic analysis indicates critical damage and derangement of eGC layer. In the ECs fraction HS-induced hypertension significantly altered protein mediators of contractility, metabolism, mechanotransduction, renal functions, and coagulation cascades. In particular, we observed dysregulation of integrin subunits alpha2, alpha2b, and alpha5, which relay external signals to the actin cytoskeleton. Conclusion: These findings provide novel molecular insight on HS-induced structure changes in eGC and ECs that may increase cardiovascular risk and potentially guide the development of new diagnostics and therapeutic interventions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:11:36.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SoFong Cam Ngan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-25 00:55:28	ID requested
1	2023-10-24 13:34:21	announced
⏵ 2	2023-11-14 09:11:40	announced	2023-11-14: Updated project metadata.

Gallart-Palau X, Lorca C, Mulet M, S, á, nchez Mil, á, n JA, Lisa J, Ngan SC, Iyappan R, Katoueezadeh M, Serra A, Sze SK, vivo to study molecular changes in each vasculature layer in murine models of disease. STAR Protoc, 4(3):102524(2023) [pubmed]

submitter keyword: cardiovascular disease

endothelial cell dysfunction

glycocalyx

high salt diet

hypertension

mass spectrometry

proteomics

Sze Sui Kwan, Newman
contact affiliation	Siu Kwan SZE, PhD, Associate professor School of Biological Sciences Division of Structural Biology and Biochemistry Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 **Current affilliation Newman S-K. Sze, PhD Professor, Department of Health Sciences CIHR Tier1 Canada Research Chair in Mechanisms of Health and Disease Brock University | Faculty of Applied Health Sciences Niagara Region | 1812 Sir Isaac Brock Way | St. Catharines, ON L2S 3A1
contact email	nsze@brocku.ca
lab head
SoFong Cam Ngan
contact affiliation	Nanyang Technological University
contact email	sfcngan@ntu.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031228
     1. Label: PRIDE project
     2. Name: Vascular bed systematic profiling unravels endothelial and glycocalyx dysregulations in experimental high salt hypertension