Updated project metadata. This project is a continuation of our previous study which resembles Interregional and Inter-hemisphere Proteome of the Human Brain. The current study aims to generate a multiregional phosphoproteome map of 12 neuroanatomical regions from both the hemisphere. The analysis of the raw data was done using MaxQuant and Perseus software. In addition to this phosphopeptides showing equal to and more than 0.75 localization probability and intensities inall the replicates were selected for further analysis. The hemisphere and region specificphosphoproteome expression has also been studied further to understand the functional aspect using in-silico tools. Finally, the data has also been integrated with the previously published proteomic expression in the brainprot (http://www.brainprot.org/) to drive the scientific community in neurobiology research.