The autophagic SNARE protein STX17 is recruited to autophagosomes and translocated to autolysosome after fusion of autophagosomes with lysosomes 30-32. We observed that the number of autophagic vacuoles with STX17 significantly increased after 2 hours of starvation. However, the number of STX17 puncta significantly decreased during prolonged starvation of 5 hours (Fig. 1a). Interestingly, no obvious degradation of STX17 was observed after prolonged starvation (Fig. 1b)