PXD031177

PXD031177 is an original dataset announced via ProteomeXchange.

Title	An optimized glycopeptide enrichment method – it’s all about the sauce
Description	Protein glycosylation is a family of post translational modifications (PTMs) that play a crucial role in cellular and tissue biology, and whose role in disease is unraveled with every new finding. Enrichment and analysis of such a diverse family of modification is very challenging, due to the number of possible glycan-peptide combinations. Among the methods used for enrichment of glycopeptides, boronic acid never lived up to its promise. While most studies focused on improving the affinity of the sugars to boronic acid, we discovered that buffer choice is as important for successful enrichment if not more. We show that amine-less buffer allows for the best enrichment results in human plasma and brain specimens. We speculate that amines compete with the glycans for boronic acid binding, therefore eliminating them improved the method significantly.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:13.194.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031177
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Morgenstern
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	hexose glycated L-lysine; complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-22 09:55:57	ID requested
1	2022-06-29 09:30:42	announced
⏵ 2	2023-11-14 08:54:13	announced	2023-11-14: Updated project metadata.

10.6019/PXD031177;

submitter keyword: glycation, Brain,Glycopeptide, PTM, Boronic acid, Plasma

Yishai Levin
contact affiliation	The Nancy and Stephen Grand Israel National Center for Personalised Medicine Weizmann Institute of Science, Rehovot 76100, Israel Tel. 972-8-9344902
contact email	yishai.levin@weizmann.ac.il
lab head
David Morgenstern
contact affiliation	Weizmann institute
contact email	david.morgenstern@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD031177

PRIDE project URI

• PRIDE
  1. PXD031177
     1. Label: PRIDE project
     2. Name: An optimized glycopeptide enrichment method – it’s all about the sauce