PXD031170

PXD031170 is an original dataset announced via ProteomeXchange.

Title	Replisome composition of stalled, terminating replication forks
Description	Topological stress can cause replication forks to stall as they converge upon one another during termination of vertebrate DNA synthesis. However, replication forks ultimately overcome topological stress and complete DNA synthesis, suggesting that alternative mechanisms can overcome topological stress. We performed a proteomic analysis of converging replication forks that were stalled by topological stress induced by loss or inhibition of topoisomerase IIα (TOP2α). Plasmid DNA was replicated in mock- or TOP2α-depleted Xenopus egg extracts as previously described (Heintzman et al. 2019). In parallel, replication was performed in the presence of the TOP2 inhibitor ICRF-193 (‘TOP2-i’) as an alternate means of preventing TOP2 activity (Heintzman et al. 2019). Chromatinized plasmid DNA was recovered 18 minutes after the onset of DNA synthesis, when most forks have normally merged but are stalled when TOP2 activity is prevented (Heintzman et al. 2019). Chromatin-bound proteins were recovered (Larsen et al. 2019) then analyzed by chromatin mass spectrometry and quantified by label free quantification.
HostingRepository	PRIDE
AnnounceDate	2023-02-21
AnnouncementXML	Submission_2023-02-20_20:46:02.674.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KristieLindsey Rose
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-22 09:47:23	ID requested
⏵ 1	2023-02-20 20:46:03	announced

Dataset with its publication pending

submitter keyword: Xenopus, LC-MSMS, topoisomerase, egg extracts, termination, DNA replication

Kristie LindseyRose
contact affiliation	Vanderbilt University Proteomics Core
contact email	kristie.rose@vanderbilt.edu
lab head
KristieLindsey Rose
contact affiliation	Vanderbilt university
contact email	kristie.rose@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031170
     1. Label: PRIDE project
     2. Name: Replisome composition of stalled, terminating replication forks