PXD031167

PXD031167 is an original dataset announced via ProteomeXchange.

Title	Comparative proteome analysis of the tegument of male and female adult Schistosoma mansoni
Description	We present a comparative analysis of sex-specific tegument proteins of paired or virgin Schistosoma mansoni. By apply a new and highly sensitive workflow, detection of even low abundance proteins from the worm was achieved Therefore, a streptavidin-biotin affinity purification technique in combination with single pot solid-phase enhanced sample preparation was established for subsequent LC-MS/MS analysis. We were able to identify 1519 tegument proteins for male and female as to virgin and paired worms. Sex-specific proteins were screened, present in both virgin and paired adult schistosomes to reduce the impact of mating. Bioinformatic analysis revealed an involvement of female-specific tegument proteins in signaling pathways of cellular processes and antioxidant mechanisms. Male-specific proteins were found to be enriched in processes linked to phosphorylation and signal transduction. This suggests a task sharing between the sexes that might be necessary for survival in the host. Our datasets provide a basis for further studies to understand and ultimately decipher the strategies of the two worm sexes to evade the immune system.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_05:52:12.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Hentschker
SpeciesList	scientific name: Schistosoma mansoni; NCBI TaxID: 6183;
ModificationList	biotinylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-21 13:21:47	ID requested
⏵ 1	2022-05-20 05:52:12	announced

Winkelmann F, Gesell Salazar M, Hentschker C, Michalik S, Mach, á, č, ek T, Scharf C, Reisinger EC, V, ö, lker U, Sombetzki M, Comparative proteome analysis of the tegument of male and female adult Schistosoma mansoni. Sci Rep, 12(1):7569(2022) [pubmed]

submitter keyword: Schistosoma manson, proteome, tegument

Martina Sombetzki
contact affiliation	Division of Tropical Medicine and Infectious Diseases, Center of Internal Medicine II, Rostock University Medical Center, Rostock, Germany
contact email	martina.sombetzki@uni-rostock.de
lab head
Christian Hentschker
contact affiliation	University Medicine Greifswald, Interfaculty Institute of Genetics and Functional Genomics, Department of Functional Genomics
contact email	christian.hentschker@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031167

PRIDE project URI

• PRIDE
  1. PXD031167
     1. Label: PRIDE project
     2. Name: Comparative proteome analysis of the tegument of male and female adult Schistosoma mansoni