PXD031165

PXD031165 is an original dataset announced via ProteomeXchange.

Title	1 Identification of replisome-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry
Description	Replication of the eukaryotic genome requires the assembly of thousands of replisomes that must work in concert to accurately replicate a cell’s genetic and epigenetic information. Defining replisome-associated proteins is a key step in understanding how genomes are replicated and repaired in the context of chromatin to maintain genome stability. To identify replisome-associated proteins, we performed iPOND (Isolation of Proteins on Nascent DNA) coupled to quantitative mass spectrometry in Drosophila embryos and cultured cells. We identified 76 and 416 replisome-associated proteins in post-MZT embryos and Drosophila cultured S2 cells, respectively . By performing a targeted screen of a subset of these proteins, we demonstrate that BRWD3, a targeting specificity factor for the DDB1/Cul4 ubiquitin ligase complex (CRL4), functions at the replisome to promote replication fork progression and maintain genome stability. Altogether, our work provides a valuable resource for those interested in the DNA replication, repair and chromatin assembly during development.
HostingRepository	PRIDE
AnnounceDate	2022-01-27
AnnouncementXML	Submission_2022-01-27_06:50:42.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Madison Wright
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-21 13:11:20	ID requested
⏵ 1	2022-01-27 06:50:43	announced

Dataset with its publication pending

submitter keyword: DNA replication, genome stability, replication fork, Drosophila, embryogenesis

Jared T. Nordman
contact affiliation	Department of Biological Sciences, Vanderbilt University, Nashville, TN 37212
contact email	jared.nordman@vanderbilt.edu
lab head
Madison Wright
contact affiliation	Vanderbilt University Department of Chemistry
contact email	madison.t.wright@vanderbilt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD031165

PRIDE project URI

• PRIDE
  1. PXD031165
     1. Label: PRIDE project
     2. Name: 1 Identification of replisome-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry