PXD031163

PXD031163 is an original dataset announced via ProteomeXchange.

Title	Identification of novel constituents of ALIS from macrophage lysates (LPS+/-), LC MS/MS
Description	Macrophages assemble aggresome-like induced structures or ALIS upon LPS or E. coli mediated TLR4 stimulation. These structures are positive for p62, LC3 and ubiquitinated proteins, form irrespective of autophagy, and persist within the cell system up to 48h. However, their composition is not well known. Our aim was to identify proteins constituting ALIS and establish its functionality in host-defense response under infectious conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:41:31.210.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031163
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Srinivasa Srinivasula
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-21 13:08:18	ID requested
1	2024-05-22 23:35:55	announced
⏵ 2	2024-10-22 06:41:35	announced	2024-10-22: Updated project metadata.

10.1091/mbc.e23-09-0347;

10.6019/PXD031163;

Bhatnagar A, Chopra U, Raja S, Das KD, Mahalingam S, Chakravortty D, Srinivasula SM, TLR-mediated aggresome-like induced structures comprise antimicrobial peptides and attenuate intracellular bacterial survival. Mol Biol Cell, 35(3):ar34(2024) [pubmed]

submitter keyword: LPS, LC MS/MS, RAW264.7, ALIS,Macrophages

Srinivasa Murty Srinivasula
contact affiliation	Laboratory of Immune Cell Biology (LICB), School of Biology, IISER Thiruvananthapuram, India
contact email	sms@iisertvm.ac.in
lab head
Srinivasa Srinivasula
contact affiliation	Indian Institute of Science Education and Research, Thiruvananthapuram
contact email	sms@iisertvm.ac.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD031163

PRIDE project URI

• PRIDE
  1. PXD031163
     1. Label: PRIDE project
     2. Name: Identification of novel constituents of ALIS from macrophage lysates (LPS+/-), LC MS/MS