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PXD031161

PXD031161 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of novel rhoptry secretion factors in Toxoplasma gondii _ ND11
DescriptionApicomplexan parasites, together with ciliates and dinoflagellates, belong to the Alveolata superphylum. Apicomplexa possess secretory organelles called rhoptries that undergo regulated exocytosis during invasion. Upon injection into the host cell, rhoptry proteins support invasion, vacuole formation, and subversion of host immune function. Rhoptry exocytosis involves a “rosette” of 8 particles embedded in the plasma membrane; such peculiar structure is also conserved in Ciliata and its formation requires Alveolata-restricted “Nd” proteins. These findings point to the existence of an Alveolata-conserved mechanism for the discharge of secretory organelles, and provide proof of concept that we can decipher rhoptry exocytosis machinery using a PAN-Alveolata approach. Thus, to uncover new rhoptry secretion factors, we used the transcriptional profiles of Nd genes of the ciliate Tetrahymena thermophila to select genes that are co-regulated, and also conserved in Apicomplexa. In this way we identified two uncharacterized Tetrahymena proteins that contribute to the exocytosis of secretory organelles. They have similar architecture and show homology with Plasmodium Cysteine Repeat Modular Proteins (CRMPs), a family of proteins essential for Plasmodium transmission from the mosquito to the host. We functionally characterized the two Toxoplasma CRMPs, including their dynamic location during invasion. They are not required for rosette formation, but they are essential for rhoptry secretion, suggesting a function distinct from that of previously characterized exocytic “Nd” factors. To characterize interacting proteins we performed pull-down experiments and analyzed the eluates by liquid chromatography-tandem mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:32:06.818.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD031161
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFunctional Proteomics Platform FPP
SpeciesList scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-21 13:06:05ID requested
12022-10-20 08:11:57announced
22023-11-14 08:32:07announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD031161;
Sparvoli D, Delabre J, Penarete-Vargas DM, Kumar Mageswaran S, Tsypin LM, Heckendorn J, Theveny L, Maynadier M, Mendon, ç, a Cova M, Berry-Sterkers L, Gu, é, rin A, Dubremetz JF, Urbach S, Striepen B, Turkewitz AP, Chang YW, Lebrun M, An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma. EMBO J, 41(22):e111158(2022) [pubmed]
Keyword List
submitter keyword: rhoptry, host invasion, secretion,Crmp, Toxoplasma
Contact List
Maryse Lebrun
contact affiliationUniversite de Montpellier LPHI UMR5235, France
contact emailmaryse.lebrun@umontpellier.fr
lab head
Functional Proteomics Platform FPP
contact affiliationFPP CNRS
contact emailcontact@fpp.cnrs.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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