PXD031160

PXD031160 is an original dataset announced via ProteomeXchange.

Title	Wild-type and SeMeCo yeast proteomes with microFlow-SWATH & DIANN
Description	Proteomic analysis of FACS-sorted and unsorted auxotrophic amd prototrophic subpopulations inSelf-establishing metabolically cooperating (SeMeCo) and control wild-type yeast communities. Microbial communities are composed of cells of varying metabolic capacity and regularly include auxotrophs; cells that lack essential metabolic pathways. By analysing auxotrophs for amino acid biosynthesis pathways in microbiome data microbiome data derived from over 12,000 natural microbial communities obtained as part of the Earth Microbiome Project (EMP), and studying auxotrophic-prototrophic interactions in self-establishing metabolically cooperating yeast communities (SeMeCos), we reveal a metabolically imprinted mechanism that links the presence of auxotrophs to an increase in metabolic interactions and gains in antimicrobial drug tolerance. As a consequence of the metabolic adaptations necessary to uptake specific metabolites, auxotrophs obtain altered metabolic flux distributions, export more metabolites, and in this way metabolite-enrich the community environments. Moreover, the increased efflux activities reduce intracellular drug concentrations, allowing cells to grow in the presence of drug levels that are above the minimal inhibitory concentrations. For example, the antifungal action of azoles is greatly diminished in auxotrophs and prototrophs that uptake metabolites from a metabolically enriched environment. Our results hence show why cells are more robust to drug exposure when they interact metabolically.
HostingRepository	PRIDE
AnnounceDate	2022-01-26
AnnouncementXML	Submission_2022-01-26_10:20:21.899.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clara Correia-Melo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-21 13:05:41	ID requested
⏵ 1	2022-01-26 10:20:22	announced

Dataset with its publication pending

submitter keyword: Sccharomyces cerevisiae, wild-type, SeMeCo, FACS auxotrophs and prototrophs, proteomics, microFlow-SWATH & DIANN

Markus Ralser
contact affiliation	The Francis Crick Institute, UK Charité – Universitätsmedizin Berlin, Germany
contact email	markus.ralser@charite.de
lab head
Clara Correia-Melo
contact affiliation	The Francis Crick Institute
contact email	clara.correia-melo@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD031160

PRIDE project URI

• PRIDE
  1. PXD031160
     1. Label: PRIDE project
     2. Name: Wild-type and SeMeCo yeast proteomes with microFlow-SWATH & DIANN