PXD031143

PXD031143 is an original dataset announced via ProteomeXchange.

Title	Haptenation of Macrophage Migration Inhibitory Factor
Description	Firstly, the adductome of the two major mouse blood proteins, as well as of the protein macrophage migration inhibitory factor (MIF) with the model hapten tetramethylrhodamine isothiocyanate (TRITC)was invastigated. After incubation at different molar ratios of TRITC, an untargeted Full MS/dd-MS2 experimental approach was used with a QExactive Quadrupole-Orbitrap mass spectrometer. The data were processed with Proteome Discoverer and the suggested sites of adductions were confirmed in parallel reaction monitoring mode (PRM) on the same instruments. Blood and lymph node samples of mise treated topically with the same hapten were screened for the peptides containing the most reactive sites of the aformentioned proteins. Only the adducted N-terminal peptide of the protein MIF could be detected. Thereafter, a quantification approach in PRM mode using standards was implemented to quantify this peptide, both modified and unmodified. Quantification was based in characteristic b and y ions.
HostingRepository	PRIDE
AnnounceDate	2022-04-08
AnnouncementXML	Submission_2022-04-08_08:33:08.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lorena Ndreu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	isothiocyanate reagent derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-21 07:38:37	ID requested
⏵ 1	2022-04-08 08:33:09	announced

Dataset with its publication pending

submitter keyword: Allergic Contact Dermatitis (ACD), Biomarkers, Contact Allergy, Macrophage Migration Inhibitory Factor (MIF), Tetramethylrhodamine isothiocyanate (TRITC), High Resolution Mass Spectrometry (HRMS)

Isabella Karlsson
contact affiliation	Stockholm University
contact email	Isabella.Karlsson@aces.su.se
lab head
Lorena Ndreu
contact affiliation	Stockholm University
contact email	lorena.ndreu@aces.su.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031143
     1. Label: PRIDE project
     2. Name: Haptenation of Macrophage Migration Inhibitory Factor