PXD031141

PXD031141 is an original dataset announced via ProteomeXchange.

Title	Antibodies to protozoan variable surface antigens induce antigenic variation
Description	The genomes of most protozoa encode families of variant surface antigens, whose mutually exclusive changes in expression allow parasitic microorganisms to evade the host immune response1. It is widely assumed that antigenic variation in protozoan parasites is accomplished by the spontaneous appearance within the population of cells expressing antigenic variants that escape antibody-mediated cytotoxicity1,2. Here we show, both in vitro and in animal infections, that antibodies to Variant-specific Surface Proteins (VSPs) of the intestinal parasite Giardia lamblia are not cytotoxic; instead, anti-VSP antibodies induce VSP clustering into microdomains, triggering a massive release of microvesicles carrying the original VSP and switching in expression to different VSPs by a calcium-dependent process. Surface microvesiculization and antigenic switching are also stimulated when Trypanosoma brucei and Tetrahymena thermophila are confronted to antibodies directed to their GPI-anchored variable surface glycoproteins. This novel mechanism of antigen clearance throughout its release into microvesicles coupled to the stochastic induction of new phenotypic variants not only changes the current paradigm of spontaneous antigenic switching but also provides a new framework for understanding the course of infections as a host/parasite adaptive process.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:16.303.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031141
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Lujan
SpeciesList	scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-21 07:35:23	ID requested
1	2023-05-10 13:17:25	announced
⏵ 2	2023-11-14 08:46:17	announced	2023-11-14: Updated project metadata.

Tenaglia AH, Luj, á, n LA, R, í, os DN, Molina CR, Midlej V, Iribarren PA, Berazategui MA, Torri A, Saura A, Peralta DO, Rodr, í, guez-Walker M, Fern, á, ndez EA, Petiti JP, Serradell MC, Gargantini PR, Sparwasser T, Alvarez VE, de Souza W, Luj, á, n HD, Antibodies to variable surface antigens induce antigenic variation in the intestinal parasite Giardia lamblia. Nat Commun, 14(1):2537(2023) [pubmed]

10.6019/PXD031141;

submitter keyword: exosomes, microvesicles,Giardia, antigenic variation

Hugo D. Lujan
contact affiliation	Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas (CIDIE), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC). Córdoba, Argentina. Facultad de Ciencias de la Salud, Universidad Católica de Córdoba (UCC). Córdoba, Argentina.
contact email	hlujan@ucc.edu.ar
lab head
Hugo Lujan
contact affiliation	CIDIE (CONICET/UCC)
contact email	hlujan@ucc.edu.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD031141

PRIDE project URI

• PRIDE
  1. PXD031141
     1. Label: PRIDE project
     2. Name: Antibodies to protozoan variable surface antigens induce antigenic variation