PXD031135
PXD031135 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Retinal pigment epithelium extracellular vesicles are potent inducers of age-related macular degeneration disease phenotype in the outer retina |
| Description | Age-related macular degeneration (AMD) is a leading cause of blindness. Vision loss is caused by the loss of the retinal pigment epithelium (RPE) and photoreceptors and/or retinal and choroidal angiogenesis. Here we use AMD patient specific RPE cells with the Y402H high-risk polymorphism in the complement factor H to perform a comprehensive analysis of EVs, their cargo and role in disease pathology. We show that AMD RPE is characterised by enhanced and polarised EV secretion. Transcriptomic, proteomic and lipidomic analyses demonstrate that AMD RPE EVs carry RNA, proteins and lipids that reflect changes in the parental RPE and mediate key AMD pathological processes including oxidative stress, cytoskeletal dysfunction, angiogenesis and drusen accumulation. We demonstrate that exposure of control RPE to AMD RPE apical EVs leads to the formation of stress vacuoles, cytoskeletal destabilization, and abnormalities in the morphology of the nucleus in the recipient cells. Treatment of retinal organoids with apical AMD RPE EVs leads to disrupted neuroepithelium and appearance of cytoprotective alpha B crystallin immunopositive cells, with some co-expressing retinal progenitor cell markers Pax6 or Vsx2, suggesting activation of regenerative pathways upon injury. These findings indicate that AMD RPE EVs mediated signalling have an important role in disease progression. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-07-11 |
| AnnouncementXML | Submission_2023-07-11_04:35:48.813.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Marzena |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-01-21 07:17:59 | ID requested | |
| ⏵ 1 | 2023-07-11 04:35:49 | announced |
Publication List
| Kurzawa-Akanbi M, Whitfield P, Burt, é F, Bertelli PM, Pathak V, Doherty M, Hilgen B, Gliaudelyt, ė L, Platt M, Queen R, Coxhead J, Porter A, Ö, berg M, Fabrikova D, Davey T, Beh CS, Georgiou M, Collin J, Boczonadi V, H, ä, rtlova A, Taggart M, Al-Aama J, Korolchuk VI, Morris CM, Guduric-Fuchs J, Steel DH, Medina RJ, Armstrong L, Lako M, Retinal pigment epithelium extracellular vesicles are potent inducers of age-related macular degeneration disease phenotype in the outer retina. J Extracell Vesicles, 11(12):e12295(2022) [pubmed] |
Keyword List
| submitter keyword: Age-related macular degeneration, Human-induced pluripotent stem cells, Retinal pigment epithelium, Complement factor H, Extracellular vesicles |
Contact List
| Majlinda Lako | |
|---|---|
| contact affiliation | Newcastle University |
| contact email | majlinda.lako@newcastle.ac.uk |
| lab head | |
| Marzena | |
| contact affiliation | Newcastle University |
| contact email | marzena.kurzawa2@newcastle.ac.uk@newcastle.ac.uk |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088713/ |
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