PXD031116 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximity biotinylation of the Toxoplasma gondii basal complex |
Description | During T. gondii cell division (endodyogeny) the basal complex acts as the cytokinetic ring by preserving daughter bud integrity and executing the tapering and basal constriction of nascent parasites. Its molecular composition, order of assembly and mode of action are incompletely understood but differ substantially from final cytokinesis events described in other organisms. In order to identify new proteins of the basal complex we used proximity biotinylation (BioID) and fused the small biotin ligase BioID2 (Kim et al., 2016) to basal complex components (BCCs). We endogenously tagged MORN1, Centrin2 (Cen2), IMC8, MyoJ, BCC1 (TGGT1_232780) and BCC2 (TGGT1_231070) with BioID2 and used an YFP-BioID2 fusion protein as a cytosolic control. Our reciprocal BioID approach identifies several novel BCCs that resolve into four basal complex sub clusters (BCSC1-4). Together with the functional characterization of critical BCCs our data uncover a novel dimension to the hierarchy and structure of daughter budding. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:15:52.006.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Klemens Engelberg |
SpeciesList | scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Discovery |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-20 09:18:06 | ID requested | |
⏵ 1 | 2024-10-08 11:15:52 | announced | |
Publication List
Keyword List
submitter keyword: Toxoplasma, basal complex, BioID |
Contact List
Marc-Jan Gubbels |
contact affiliation | Boston College, Department of Biology, MA, USA |
contact email | gubbelsj@bc.edu |
lab head | |
Klemens Engelberg |
contact affiliation | Boston College, Department of Biology |
contact email | klemens.engelberg@bc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031116 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031116
- Label: PRIDE project
- Name: Proximity biotinylation of the Toxoplasma gondii basal complex