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PXD031107

PXD031107 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteogenomic analysis of relapsed acute myeloid leukemia in adults and children
DescriptionDespite improvement of current treatment strategies and novel targeted drugs, relapse and treatment resistance determine the major cause of death for acute myeloid leukemia (AML) patients. To identify the underlying molecular characteristics, numerous studies have been aimed to decipher the genomic- and transcriptomic landscape of AML. Nevertheless, further molecular changes allowing malignant cells to escape treatment are yet to be elucidated. Mass spectrometry is a powerful tool enabling detailed insights into proteomic changes that could explain AML relapse and resistance. Here, we investigated AML samples from 47 adult and 22 pediatric patients at serial time-points during disease progression using high resolution isoelectric focusing liquid chromatography mass spectrometry. We show that the proteomic profile at relapse is enriched for mitochondrial ribosomal proteins and subunits of the respiratory chain complex, indicative of reprogrammed energy metabolism from diagnosis to relapse. Further, higher levels of granzymes and lower levels of the anti-inflammatory protein CR1/CD35 suggest an inflammatory signature promoting disease progression. Finally, through a proteogenomic approach, we detected novel peptides, which present a promising repertoire in the search for biomarkers and tumor-specific druggable targets. Altogether, this study highlights the importance of proteomic studies in holistic approaches to improve treatment and survival of AML patients.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:07:36.421.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMattias Vesterlund
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-20 09:02:12ID requested
12023-01-09 18:51:45announced
22023-11-14 08:07:37announced2023-11-14: Updated project metadata.
Publication List
Stratmann S, Vesterlund M, Umer HM, Eshtad S, Skaftason A, Herlin MK, Sundstr, ö, m C, Eriksson A, H, ö, glund M, Palle J, Abrahamsson J, Jahnukainen K, Munthe-Kaas MC, Zeller B, Tamm KP, Lindskog C, Cavelier L, Lehti, ö J, Holmfeldt L, Proteogenomic analysis of acute myeloid leukemia associates relapsed disease with reprogrammed energy metabolism both in adults and children. Leukemia, 37(3):550-559(2023) [pubmed]
Keyword List
submitter keyword: pediatric, Myeloblast, Human, LC-MS,Leukemia, Cancer, Proteogenomics, Clinical samples., HiRIEF, adult, Proteomics, Acute Myeloid Leukemia
Contact List
Janne Lehtiö
contact affiliationDepartment of Oncology-Pathology, Karolinska Institutet and Science for Life Laboratory, Stockholm, Sweden
contact emailjanne.lehtio@ki.se
lab head
Mattias Vesterlund
contact affiliationKarolinska Institutet
contact emailmattias.vesterlund@ki.se
dataset submitter
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Dataset FTP location
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