PXD031106

PXD031106 is an original dataset announced via ProteomeXchange.

Title	Detection of putative PKA target phosphorylation sites in Atg5flox:CamKII-Cre wildtype and knockout mice brain tissue
Description	The experiment was performed to identify PKA phosphorylation substrates in wildtype and autophagy-deficient brains. Therefore, hippocampus and cortex of wildtype and conditional knockout mice were isolated and sliced. The brain slices were incubated with DMSO or Forskolin, a cAMP elevating agent, to induce PKA activity. Samples were measured by LC-MS/MS and were used to quantify proteomic and phosphoproteomic changes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:36:33.899.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederik Tellkamp
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-20 06:24:01	ID requested
1	2022-11-15 06:55:06	announced
⏵ 2	2023-11-14 07:36:34	announced	2023-11-14: Updated project metadata.

Overhoff M, Tellkamp F, Hess S, Tolve M, Tutas J, Faerfers M, Ickert L, Mohammadi M, De Bruyckere E, Kallergi E, Delle Vedove A, Nikoletopoulou V, Wirth B, Isensee J, Hucho T, Puchkov D, Isbrandt D, Krueger M, Kloppenburg P, Kononenko NL, Autophagy regulates neuronal excitability by controlling cAMP/protein kinase A signaling at the synapse. EMBO J, 41(22):e110963(2022) [pubmed]

submitter keyword: Forskolin, autophagy-deficiency,Brain slices, PKA signalling

Natalia L Kononenko
contact affiliation	CECAD Excellence Center & Center for Physiology, Medical Faculty, University of Cologne, Germany
contact email	n.kononenko@uni-koeln.de
lab head
Frederik Tellkamp
contact affiliation	University of Cologne, Department of Biology, Institute for Genetics
contact email	frederik.tellkamp@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD031106

PRIDE project URI

• PRIDE
  1. PXD031106
     1. Label: PRIDE project
     2. Name: Detection of putative PKA target phosphorylation sites in Atg5flox:CamKII-Cre wildtype and knockout mice brain tissue