PXD031104

PXD031104 is an original dataset announced via ProteomeXchange.

Title	DIA-based proteome analysis reveals the protective role of Nano-selenium against heat stress in rainbow trout
Description	With the continuous intensification of global warming, extreme climate fluctuations and high-density farming, heat stress severely restrict the survival and yield of the world economic cold-water rainbow trout (Oncorhynchus mykiss). With the development of nanotechnology, Nano-selenium (Nano-Se) has become an ideal and ecological Se nutrient source additive and free radical scavenger with its outstanding advantages of high biological activity and low toxicity. Herein, fish were fed to two levels Nano-Se at 18°C for 9 days: CG18 (0 mg/kg) and Se18 (5 mg/kg). Afterward, the temperature of each group was raised to 24°C and exposed for 8 h (CG24, Se24). DIA/SWATH technique was used to analyze the proteome changes in rainbow trout liver under heat stress (CG18-CG24) and used this process as a control, focusing on comparison the proteome changes in heat stress after the addition of Nano-Se (Se18-Se24). Results showed that 223 and 269 differentially abundant proteins in CG18-CG24 and Se18-Se24, respectively, mainly including apoptosis, heat shock protein (HSPs), selenoprotein and lipid-related proteins. Compared with the CG18-CG24 group, the Se18-Se24 group increased the up-regulated abundance of HSPs (Hsp70, Hsp90a.1, Hspa8, Hsp30, DNAJA4 and DNAJB1); partially restored the down-regulated abundance of selenoprotein (MsrB2); significantly down-regulated the abundance of lipid-related protein (CYP51, EBP, DHCR7, DHCR24 and APOB) and decreased the abundance of pro-apoptotic protein (Caspase8 and Bad). PPI results indicated that Nano-Se may inhibit apoptosis through up-regulating Hsp70, Hsp90a.1, Hspa8 and DNAJb1, and Hsp70/Hspa8 and MsrB2 may synergistically exert antioxidant defense effects under heat stress. Overall, our results demonstrated that Nano-Se takes protective roles in rainbow trout against heat stress by strengthening protein repair, recovering antioxidant enzyme activity, alleviating lipid metabolism and improving apoptosis. The present study provided novel insights into Nano-Se- mediated tolerance of rainbow trout against heat stress at the proteomic level.
HostingRepository	iProX
AnnounceDate	2022-01-19
AnnouncementXML	Submission_2022-01-19_18:15:46.945.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Liu
SpeciesList	scientific name: Oncorhynchus mykiss; NCBI TaxID: 8022;
ModificationList	methionine oxidation with neutral loss of 64 Da
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-19 18:15:33	ID requested
⏵ 1	2022-01-19 18:15:47	announced

Dataset with its publication pending

submitter keyword: Heat stress, Nano-selenium, DIA/SWATH based proteomic, Heat shock protein, Selenoprotein, Oncorhynchus mykiss

Zhe Liu
contact affiliation	Gansu Agricultural University
contact email	liuz@gsau.edu.cn
lab head
Zhe Liu
contact affiliation	Gansu Agricultural University
contact email	liuz@gsau.edu.cn
dataset submitter

iProX dataset URI