PXD031094

PXD031094 is an original dataset announced via ProteomeXchange.

Title	Pan-claudin family interactome analysis reveals shared and specific interactions
Description	Interactome study of the pan-claudin family by combining two complementary pull-down techniques followed by mass spectrometry to create an interaction landscape of the claudin family. Co-immunoprecipitation (CoIP) of recombinant claudins expressed in MDCK-C7 cells provided information about interactions beyond the already known TJ proteins. A protein interaction screen on a peptide matrix (PRISMA) allowed mapping interactions along the disordered cytosolic C-terminal region of claudins and studying the effect of post-translational modifications (PTMs) on these interactions. The new interaction partners of the C-terminus of several claudins were confirmed by proximity ligation assays (PLA) and revealed their possible implications in localized biological processes in epithelial cells. By combining these approaches, we created an extended interaction map of the claudin protein family, expanding the current knowledge in the field.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:09:43.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lorena Suarez-Artiles
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-19 09:33:01	ID requested
1	2023-10-24 12:22:35	announced
⏵ 2	2023-11-14 09:09:45	announced	2023-11-14: Updated project metadata.

Perez-Hernandez D, Suarez-Artiles L, Jones MSO, Dittmar G, Using PrISMa to reveal the interactome of the human claudins family. STAR Protoc, 4(4):102549(2023) [pubmed]

submitter keyword: LC-MS/MS, PRISMA,Claudin, Pulldown, Interactome, Peptide pulldown

Gunnar Dittmar
contact affiliation	Proteomics of cellular signaling, Luxembourg Institute of Health, Strassen, Luxembourg. Department of Life Sciences and Medicine, University ofLuxembourg, Campus Belval, Luxembourg.
contact email	gunnar.dittmar@lih.lu
lab head
Lorena Suarez-Artiles
contact affiliation	Max-Delbrueck Center for Molecular Medicine
contact email	Lorena.suarez@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD031094

PRIDE project URI

• PRIDE
  1. PXD031094
     1. Label: PRIDE project
     2. Name: Pan-claudin family interactome analysis reveals shared and specific interactions