PXD031078

PXD031078 is an original dataset announced via ProteomeXchange.

Title	Proteomic Investigation of Evolved Daptomycin-Tolerant MRSA due to Mutation in YycH
Description	Several groups have shown that through evolution experiments, tolerance and resistance evolved rapidly under cyclic antibiotic treatment. In other words, intermittent antibiotic exposure performed in a typical adaptive laboratory evolution (ALE) experiments will “train” the bacteria to become tolerant/resistant to the drug. Although ALE has added new knowledge regarding the impact of varying treatment conditions on the evolution of tolerance/resistance, the role of some parameters such as population bottlenecks remains poorly understood. In this study, we employed ALE to investigate the evolution of methicillin-resistant S. aureus under repetitive daptomycin treatment using a modified protocol that incorporated population bottleneck following antibiotic exposure. We observed that although tolerance development is slower under bottlenecking conditions, the populations finally attained tolerance mutation in the yycH gene after twelve cycles of treatment. Extending the evolution experiment and changing the treatment scheme to a fast evolution protocol (treatment during exponential phase without bottlenecking) led to the emergence of daptomycin resistance (mutation in mprF gene). Through proteomics, we uncovered the differential adaptation strategies of these daptomycin tolerant and resistant MRSA strains, and how they respond differently to antibiotics compared to the ancestral wild-type.
HostingRepository	PRIDE
AnnounceDate	2022-07-16
AnnouncementXML	Submission_2022-07-15_21:11:54.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordy Evan Sulaiman
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-19 03:06:41	ID requested
⏵ 1	2022-07-15 21:11:54	announced

Dataset with its publication pending

submitter keyword: Methicillin-resistant S. aureus, daptomycin, tolerance, adaptive laboratory evolution, proteomics, yycH

Henry Lam
contact affiliation	Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST)
contact email	kehlam@ust.hk
lab head
Jordy Evan Sulaiman
contact affiliation	The Hong Kong University of Science and Technology (HKUST)
contact email	jesulaiman@connect.ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD031078

PRIDE project URI

• PRIDE
  1. PXD031078
     1. Label: PRIDE project
     2. Name: Proteomic Investigation of Evolved Daptomycin-Tolerant MRSA due to Mutation in YycH