PXD031067

PXD031067 is an original dataset announced via ProteomeXchange.

Title	The fate of bioaccessible coeliac toxic motifs and IgE epitopes during simulated gastro-intestinal digestion of pizza base
Description	Understanding how food processing may modify allergen bioaccessibility and the evolution of immunologically active peptides in the gastrointestinal tract is essential if knowledge-based ap-proaches to reducing the allergenicity of food is to be realised. A soy-enriched wheat-based pizza base was subjected to in vitro oral-gastro-duodenal digestion and resulting digests analysed using a combination of SDS-PAGE and mass spectrometry (MS). The digestion profile of pizza base resembled that of bread crust where higher temperatures during baking reduced protein solu-bility but still resulted in the generation of a complex mixture of peptides. MS profiling showed of the peptides carried IgE-epitopes and coeliac toxic motifs many of which were in excess of 20-30 residues long which were only released after extended (120 min) gastric digestion or a combina-tion of gastric and duodenal digestion. Comparison with in silico prediction tools showed that they either under-estimated pepsinolysis products or over estimated tryptic/chymotryptic cleavage patterns. These data show that simple thermal treatments, such as baking, are insuffi-cient to destroy the allergenic potential of wheat and soybean proteins. The findings contribute to efforts to provide bench marks for mapping in vitro digestion products of novel proteins as part of the allergenicity risk assessment.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:37:45.082.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Daly
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	monohydroxylated residue; monohydroxylated proline; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-19 02:40:28	ID requested
⏵ 1	2022-08-11 23:37:45	announced

Daly ME, Wang K, Pan X, Depau RL, Marsh J, Capozzi F, Johnson P, Gethings LA, Mills ENC, The Fate of IgE Epitopes and Coeliac Toxic Motifs during Simulated Gastrointestinal Digestion of Pizza Base. Foods, 11(14):(2022) [pubmed]

submitter keyword: Wheat, gluten, soy, allergen, coeliac toxic motif, IgE epitope, in vitro digestion, mass spectrome-try, allergenicity risk assessment.

Clare Mills
contact affiliation	Manchester Institute of Biotechnology, School of Biological Sciences, Manchester Academic Health Sciences Centre, The University of Manchester, Princess Street, Manchester, M1 7DN, UK
contact email	clare.mills@manchester.ac.uk
lab head
Matthew Daly
contact affiliation	University of Manchester
contact email	matthew.daly-3@postgrad.manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031067
     1. Label: PRIDE project
     2. Name: The fate of bioaccessible coeliac toxic motifs and IgE epitopes during simulated gastro-intestinal digestion of pizza base