PXD031057

PXD031057 is an original dataset announced via ProteomeXchange.

Title	Leishmania braziliensis Protein Arginine Methyltransferases interactomes
Description	We functionally characterized the five predicted PRMTs in Leishmania braziliensis by gene knockout and endogenous protein HA tagging using CRISPR/Cas9 gene editing. We report that R-methylation profiles vary among Leishmania species and across L. braziliensis lifecycle stages, with the peak PRMT expression occurring in promastigotes. A list of PRMT-interacting proteins was obtained in a single coimmunoprecipitation assay using HA-tagged PRMTs, suggesting a network of putative targets of PRMTs and cooperation between the R-methylation writers. Knockout of each L. braziliensis PRMT led to significant changes in global arginine methylation patterns without affecting cell viability. Deletion of either PRMT1 or PRMT3 disrupted most type I PRMT activity, resulting in a global increase in monomethyl arginine levels. Using anti-MMA antibodies, we performed an IP experiment to identify MMA proteins in the parental line, single PRMT1 knockout, PRMT1/PRMT7 double knockout and PRMT1-Addback parasites. The results indicate that R-methylation is modulated across lifecycle stages in L. braziliensis and show possible functional overlap and cooperation among the different PRMTs in targeting proteins. Overall, our data suggest important regulatory roles of these proteins throughout the L. braziliensis life cycle, showing that arginine methylation is important for parasite-host cell interactions. Linked publication: https://doi.org/10.1101/2021.09.22.461376.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:34.711.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031057
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lissur Orsine
SpeciesList	scientific name: Leishmania braziliensis; NCBI TaxID: 5660;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-18 02:35:11	ID requested
1	2022-03-03 07:35:01	announced
⏵ 2	2023-11-14 08:54:35	announced	2023-11-14: Updated project metadata.

10.6019/PXD031057;

submitter keyword: Leishmania braziliensis, PRMT, protein arginine methyltransferase, gene knockout, CRISPR/Cas9

Angela Kaysel Cruz
contact affiliation	Molecular Parasitology Laboratory, Cell and Molecular Biology Department, Ribeirão Preto Medical School, University of São Paulo, Brazil
contact email	akcruz@fmrp.usp.br
lab head
Lissur Orsine
contact affiliation	Molecular Parasitology Laboratory, Cell and Molecular Biology Department, Ribeirão Preto Medical School, University of São Paulo, Brazil
contact email	lissurorsine@fmrp.usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031057
     1. Label: PRIDE project
     2. Name: Leishmania braziliensis Protein Arginine Methyltransferases interactomes