PXD031056

PXD031056 is an original dataset announced via ProteomeXchange.

Title	Inflammatory Activation Drives Re-Sculpting and Shear-Thinning of the Dendritic Cell Nucleus
Description	To mount an adaptive immune response, dendritic cells must migrate to lymph nodes to present antigens to T cells. Critical to 3D migration is the nucleus, which is the size-limiting barrier for migration through the extracellular matrix. Here, we show that inflammatory activation of dendritic cells leads to the nucleus becoming spherically deformed and enables dendritic cells to overcome the typical 2- to 3-μm diameter limit for 3D migration through gaps in the extracellular matrix. We show that the nuclear shape change is partially attained through reduced cell adhesion, whereas improved 3D migration is achieved through reprogramming of the actin cytoskeleton. Specifically, our data point to a model whereby the phosphorylation of cofilin-1 at serine 41 drives the assembly of a cofilin-actomyosin ring proximal to the nucleus and enhances migration through 3D collagen gels. In summary, these data describe signaling events through which dendritic cells deform their nucleus and enhance their migratory capacity.
HostingRepository	PRIDE
AnnounceDate	2024-03-14
AnnouncementXML	Submission_2024-03-14_01:08:40.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giulia Franciosa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-18 02:34:01	ID requested
⏵ 1	2024-03-14 01:08:41	announced

10.1016/j.celrep.2024.113866;

Warner H, Franciosa G, van der Borg G, Coenen B, Faas F, Koenig C, de Boer R, Classens R, Maassen S, Baranov MV, Mahajan S, Dabral D, Bianchi F, van Hilten N, Risselada HJ, Roos WH, Olsen JV, Cano LQ, van den Bogaart G, Atypical cofilin signaling drives dendritic cell migration through the extracellular matrix via nuclear deformation. Cell Rep, 43(3):113866(2024) [pubmed]

submitter keyword: Dendritic cells, LC-MS/MS

Giulia Franciosa
contact affiliation	Novo Nordisk Foundation Center for Protein Research Proteomics Program University of Copenhagen Faculty of Health and Medical Sciences Blegdamsvej 3b DK-2200 Copenhagen Denmark
contact email	giulia.franciosa@cpr.ku.dk
lab head
Giulia Franciosa
contact affiliation	Novo Nordisk Foundation Center For Protein Research, Copenhagen University, Copenhagen
contact email	giulia.franciosa@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD031056

PRIDE project URI

• PRIDE
  1. PXD031056
     1. Label: PRIDE project
     2. Name: Inflammatory Activation Drives Re-Sculpting and Shear-Thinning of the Dendritic Cell Nucleus