PXD031043

PXD031043 is an original dataset announced via ProteomeXchange.

Title	The proteome of cytomatrix and cytosol
Description	The cytoplasm is an essential part of the cell involved in protein synthesis, energy generation, nutrient processing, transport, metabolism, and homeostasis, but its structure and function are not entirely understood. Almost five decades ago, dynamic nano-compartments of the cytoplasm were observed by high voltage electron microscopy in every living cell. However, the validity of these constructs, have been questioned and counterclaimed as artifacts of histo-techniques. We developed a new method to separate and characterize two different cytoplasmic compartments that we named cytomatrix and cytosol (or cytomedia). Using two compositional different solvents we were able sequentially extract the cytosol (cyto-solution) first then separate the cytomatrix from the nucleus. Our method allows fractionate the cell into three fractions: the cytosol, cytomatrix, and the nucleus.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:44.412.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nelufa Islam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-18 02:07:39	ID requested
1	2023-03-11 04:38:15	announced
⏵ 2	2023-11-14 08:42:49	announced	2023-11-14: Updated project metadata.

Shaiken TE, Grimm SL, Siam M, Williams A, Rezaeian AH, Kraushaar D, Ricco E, Robertson MJ, Coarfa C, Jain A, Malovannaya A, Stossi F, Opekun AR, Price AP, Dubrulle J, Transcriptome, proteome, and protein synthesis within the intracellular cytomatrix. iScience, 26(2):105965(2023) [pubmed]

submitter keyword: cytosol, cytoplasm, mass-spectrometry, high throughput analysis, cytomedia, cytomatrix,proteome

Tattym E Shaiken
contact affiliation	Senior Staff Scientist Department of Medicine -Gastroenterology and Hepatology Baylor College of Medicine Houston, TX US
contact email	tattym.shaiken@bcm.edu
lab head
Nelufa Islam
contact affiliation	Baylor College of Medicine
contact email	nelufa.islam@bcm.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD031043

PRIDE project URI

• PRIDE
  1. PXD031043
     1. Label: PRIDE project
     2. Name: The proteome of cytomatrix and cytosol