Update publication information. We used SPIDER to examine the cell-surface receptors for specific ligands while these receptors were in their native membranes within intact cells in culture. Here we coupled the SPIDER assay with biotinylated RBD of the Omicron Variant of SARS-CoV-2 and biotinylated RBD of SARS-CoV-2. Then we performed mass spectrometry strategy to identify novel binding proteins on the surface of VERO E6,Calu-3,and H1299 cells.