PXD031034

PXD031034 is an original dataset announced via ProteomeXchange.

Title	Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid nanocarriers during blood-brain barrier crossing
Description	Upon entering in contact with the biological environment, nanostructures are immediately covered by biomolecules, and in particular proteins forming the so-called “protein corona” (PC). The phenomenon of PC formation has gained great attention in recent years due to its implication for the use of nanostructures in biomedicine. In fact, it has been shown that the formation of the PC can impact the performance of nanostructures by reducing their stability, causing aggregation, increasing their toxicity, and causing unexpected and undesired nanostructure-cell interactions. In this work, we decided to study for the first time the formation and the evolution of PC on the surface of nanostructured lipid carriers loaded with superparamagnetic iron oxide nanoparticles (SPIONs), before and after the crossing of an in vitro model of the blood-brain barrier. Combining confocal microscopy, direct STochastic Optical Reconstruction Microscopy (dSTORM), and proteomic analysis, we were able to provide a complete analysis of the PC formation and evolution. In particular, we evidence that PC formation is a fast process, being formed around particles even after just 1 min of exposure to fetal bovine serum. Moreover, PC formed around particles is extremely heterogeneous: while some particles have no associated PC at all, others are completely covered by proteins. Lastly, the interaction with an in vitro blood-brain barrier model strongly affects the PC composition: in particular, a large amount of the proteins forming the initial PC is lost after the BBB passage and is partially replaced by new proteins derived from both the brain endothelial cells and from the cell culture medium. Altogether, the presented data could potentially provide new insights into the design and fabrication of lipid nanostructures for the treatment of central nervous system disorders.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:25:33.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Bartolucci
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-17 08:58:33	ID requested
1	2022-10-14 11:05:52	announced
⏵ 2	2023-11-14 07:25:35	announced	2023-11-14: Updated project metadata.

Battaglini M, Feiner N, Tapeinos C, De Pasquale D, Pucci C, Marino A, Bartolucci M, Petretto A, Albertazzi L, Ciofani G, Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing. Nanoscale, 14(36):13292-13307(2022) [pubmed]

submitter keyword: Protein corona

blood-brain barrier

lipid nanoparticles

magnetic nanoparticles

super resolution microscopy

dSTORM

mass spectroscopy.

Andrea Petretto
contact affiliation	IRCCS Istituto Giannina Gaslini
contact email	a.petretto@gmail.com
lab head
Martina Bartolucci
contact affiliation	IRCCS Gaslini
contact email	smartibartolucci@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD031034

PRIDE project URI

• PRIDE
  1. PXD031034
     1. Label: PRIDE project
     2. Name: Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid nanocarriers during blood-brain barrier crossing