PXD031009

PXD031009 is an original dataset announced via ProteomeXchange.

Title	RNA-protein interactome reveals essential roles of RPL5 and RNA helicase A in mediating translation from the Hepatitis E virus internal ribosome entry site-like element
Description	Multiple processes exist in a cell to ensure continuousproduction of essential proteins either through cap-dependent or cap-independent translation processes. Viruses depend on the host translation machinery for viral protein synthesis. Therefore, viruses have evolved clever strategies to utilize the host translation machinery. Earlier studies have shown that genotype1 hepatitis E virus (g1-HEV) utilizes both cap-dependent and cap-independent translation machineries for its replication and proliferation. Cap-independent translation in g1-HEV is driven by an eighty seven nucleotide-long RNA element which acts as a noncanonical, internal ribosome entry site like (IRESl) element. Here, we have identified the RNA-protein interactome of the HEV IRESl element and characterized the functional significance of some of its components. Our study reveals indispensable roles of host ribosomal proteinRPL5 and DHX9 (RNA helicase A) in mediating efficient translation from the IRESlelement and establish the function of HEV IRESl as a bonafide internal ribosome entry site.
HostingRepository	PRIDE
AnnounceDate	2023-06-25
AnnouncementXML	Submission_2023-06-25_01:02:21.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MIlan Surjit
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-16 23:03:43	ID requested
⏵ 1	2023-06-25 01:02:21	announced

Dataset with its publication pending

submitter keyword: Hepatitis E virus;cap-independent translation

RNA-protein interaction

Virus-host interaction;internal ribosome entry site, HEV ORF4.

Dr. MilanSurjit
contact affiliation	Molecular virology, THSTI, NCR Biotech Science Cluster, Faridabad, India
contact email	milan@thsti.res.in
lab head
MIlan Surjit
contact affiliation	Translational of health science and technology institute, India.
contact email	milan@thsti.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031009
     1. Label: PRIDE project
     2. Name: RNA-protein interactome reveals essential roles of RPL5 and RNA helicase A in mediating translation from the Hepatitis E virus internal ribosome entry site-like element