PXD031007

PXD031007 is an original dataset announced via ProteomeXchange.

Title	Genetically encoding histone mark for mapping the chromatin interactomes via living cell proximity labeling
Description	We developed a method using site-specific genetic code expansion strategy in conjunction with APEX2-mediated proximity labeling to decipher interactors of hPTMs in living cells via quantitative proteomics. The method allows site-specific incorporation of a PTM-modified amino acid into histones that fused to APEX2, facilitating the labeling of the PTM-modified histones to binding proteins in vivo. Thus, this system will be a promising alternative strategy for interpreting the interactomes of hPTMs in cellular contexts.
HostingRepository	iProX
AnnounceDate	2022-01-14
AnnouncementXML	Submission_2022-01-16_23:03:11.443.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yepei Huang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-16 23:02:19	ID requested
⏵ 1	2022-01-16 23:03:11	announced

Dataset with its publication pending

submitter keyword: hPTM interactomes, proximity labeling, genetic code expansion strategy, histone crotonylation, quantitative proteomics

Kai Zhang
contact affiliation	Tianjin Medical University
contact email	kaizhang@tmu.edu.cn
lab head
Yepei Huang
contact affiliation	Tianjin Medical University
contact email	huangyepei@tmu.edu.cn
dataset submitter

iProX dataset URI