PXD031002

PXD031002 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein expression landscape defines the formation potential of mouse blastoids from EPS cells
Description	Preimplantation embryo development is a precisely regulated process organized by maternally inherited and newly synthesized proteins. Recently, some studies have reported that blastocyst-like structures, named blastoids, can be generated from mouse ESCs (embryonic stem cells) or EPSCs (extended pluripotent stem cells). In this study, to explore the dynamic expression characteristics of proteins and their PTMs in mouse EPS blastoids, we revealed the protein expression profile of EPS-blastoids and metabolite characteristics by TMT-based quantitative mass spectrometry (MS) strategy. Furthermore, the protein phosphorylation sites were identified to show the phosphoproteomic analysis in blastoids compared with mouse early embryos. Above all, our study revealed the protein expression profile of EPS blastoids compared with mouse embryos during preimplantation development and indicated that glucose metabolism is key to blastoid formation.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_09:13:55.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheying Min
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-16 22:53:49	ID requested
⏵ 1	2022-05-25 09:14:01	announced

Publication List

Min Z, Zhong K, Luo Y, Fan Y, Yu Y, Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs. Front Cell Dev Biol, 10():840492(2022) [pubmed]

Min Z, Zhong K, Luo Y, Fan Y, Yu Y, Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs. Front Cell Dev Biol, 10():840492(2022) [pubmed]

Keyword List

submitter keyword: blastoids； mEPSCs； proteome； glucose metabolism； phosphorylation； post translation modifications

submitter keyword: blastoids； mEPSCs； proteome； glucose metabolism； phosphorylation； post translation modifications

Contact List

Zheying Min
contact affiliation	The Third Affiliated Hospital of Guangzhou Medical University, Peking University Third Hospital.
contact email	minzheer@163.com
lab head
Zheying Min
contact affiliation	The Third Affiliated Hospital of Guangzhou Medical University
contact email	minzheer@163.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD031002
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD031002

PRIDE project URI

Repository Record List

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