PXD030998

PXD030998 is an original dataset announced via ProteomeXchange.

Title	Metabolic profiling of interspecies interactions during sessile bacterial cultivation reveals growth and sporulation induction in Paenibacillus amylolyticus in response to Xanthomonas retroflexus - Samples from Paenibacillus amylolyticus
Description	An in-depth characterization of an observed interspecies interaction between two co-isolated bacteria, Xanthomonas retroflexus and Paenibacillus amylolyticus. Using microsensor measurements for mapping the chemical environment, we show how X. retroflexus promoted an alkalization of its local environment through degradation of amino acids and release of ammonia. When the two species were grown in proximity, the modified local environment induced a morphological change and increased growth of P. amylolyticus followed by sporulation. 2D spatial metabolomics enabled visualization and mapping of the degradation of oligopeptide structures by X. retroflexus and morphological changes of P. amylolyticus through e.g. the release of membrane-associated metabolites. Proteome analysis and microscopy were used to validate the shift from vegetative growth towards sporulation. In summary, we demonstrate how environmental profiling by combined application of microsensor, microscopy, metabolomics and proteomics approaches can reveal growth and sporulation promoting effects resulting from interspecies interactions.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_05:44:05.128.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jakob Herschend
SpeciesList	scientific name: Paenibacillus amylolyticus; NCBI TaxID: 1451;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-16 13:33:20	ID requested
⏵ 1	2022-05-20 05:44:05	announced

Herschend J, Ernst M, Koren K, Melnik AV, da Silva RR, R, ø, der HL, Damholt ZBV, H, ä, gglund P, Svensson B, S, ø, rensen SJ, K, ü, hl M, Dorrestein PC, Burm, ø, lle M, . Front Cell Infect Microbiol, 12():805473(2022) [pubmed]

submitter keyword: LC-MSMS, Bacteria, Interspecies interactions sporulation

Mette Burmølle
contact affiliation	University of Copenhagen, Section of Microbiology
contact email	Burmolle@bio.ku.dk
lab head
Jakob Herschend
contact affiliation	University of Copenhagen
contact email	jakobherschend@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030998

PRIDE project URI

• PRIDE
  1. PXD030998
     1. Label: PRIDE project
     2. Name: Metabolic profiling of interspecies interactions during sessile bacterial cultivation reveals growth and sporulation induction in Paenibacillus amylolyticus in response to Xanthomonas retroflexus - Samples from Paenibacillus amylolyticus