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PXD030994

PXD030994 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamic changes in the proteome of early bovine embryos developed in vivo
DescriptionEarly embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and first cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed in vivo. Eleven Holstein females were used as embryo donors and pools of embryos at the 4-6 cell, 8-12 cell, morula, compact morula and blastocyst stages were analyzed by nanoliquid chromatography coupled with tandem MS (nanoLC-MS/MS). A total of 2757 proteins were identified, of which 1950 were quantitatively analyzed. Principal component analysis of data showed a separation of embryo pools according to their developmental stage. Differentially abundant proteins (DAPs) between stages were clustered into 626 upregulated and 400 downregulated proteins with most significant changes at the time of EGA and blastocyst formation. The main pathways and processes overrepresented among upregulated proteins were RNA metabolism, protein translation and ribosome biogenesis whereas Golgi vesicle transport and protein processing in endoplasmic reticulum were overrepresented among downregulated proteins. This is the first comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo. These data provide a number of functional protein candidates and will be useful to evaluate the impact of in vitro conditions on embryo quality.
HostingRepositoryPRIDE
AnnounceDate2022-05-20
AnnouncementXMLSubmission_2022-05-20_05:40:30.914.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD030994
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterE Com
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-16 13:11:01ID requested
12022-05-20 05:40:31announced
Publication List
Banliat C, Mah, é C, Lavigne R, Com E, Pineau C, Labas V, Guyonnet B, Mermillod P, Saint-Dizier M, . Front Cell Dev Biol, 10():863700(2022) [pubmed]
Keyword List
submitter keyword: Embryo, proteomics, mass spectrometry, morula, blastocyst, cattle, development.
Contact List
Charles Pineau
contact affiliationUMS Biosit - CNRS 3480 - Inserm 018 Campus de Beaulieu - Bâtiment. 24 - CS2407 263 Avenue du Général Leclerc 35042 RENNES CEDEX FRANCE
contact emailproteome@univ-rennes1.fr
lab head
E Com
contact affiliationProtim, Irset, Inserm U1085
contact emailemmanuelle.com@univ-rennes1.fr
dataset submitter
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Dataset FTP location
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