PXD030994

PXD030994 is an original dataset announced via ProteomeXchange.

Title	Dynamic changes in the proteome of early bovine embryos developed in vivo
Description	Early embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and first cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed in vivo. Eleven Holstein females were used as embryo donors and pools of embryos at the 4-6 cell, 8-12 cell, morula, compact morula and blastocyst stages were analyzed by nanoliquid chromatography coupled with tandem MS (nanoLC-MS/MS). A total of 2757 proteins were identified, of which 1950 were quantitatively analyzed. Principal component analysis of data showed a separation of embryo pools according to their developmental stage. Differentially abundant proteins (DAPs) between stages were clustered into 626 upregulated and 400 downregulated proteins with most significant changes at the time of EGA and blastocyst formation. The main pathways and processes overrepresented among upregulated proteins were RNA metabolism, protein translation and ribosome biogenesis whereas Golgi vesicle transport and protein processing in endoplasmic reticulum were overrepresented among downregulated proteins. This is the first comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo. These data provide a number of functional protein candidates and will be useful to evaluate the impact of in vitro conditions on embryo quality.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_05:40:30.914.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030994
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	E Com
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-16 13:11:01	ID requested
⏵ 1	2022-05-20 05:40:31	announced

Banliat C, Mah, é C, Lavigne R, Com E, Pineau C, Labas V, Guyonnet B, Mermillod P, Saint-Dizier M, . Front Cell Dev Biol, 10():863700(2022) [pubmed]

submitter keyword: Embryo, proteomics, mass spectrometry, morula, blastocyst, cattle, development.

Charles Pineau
contact affiliation	UMS Biosit - CNRS 3480 - Inserm 018 Campus de Beaulieu - Bâtiment. 24 - CS2407 263 Avenue du Général Leclerc 35042 RENNES CEDEX FRANCE
contact email	proteome@univ-rennes1.fr
lab head
E Com
contact affiliation	Protim, Irset, Inserm U1085
contact email	emmanuelle.com@univ-rennes1.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030994
     1. Label: PRIDE project
     2. Name: Dynamic changes in the proteome of early bovine embryos developed in vivo