PXD030994 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dynamic changes in the proteome of early bovine embryos developed in vivo |
Description | Early embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and first cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed in vivo. Eleven Holstein females were used as embryo donors and pools of embryos at the 4-6 cell, 8-12 cell, morula, compact morula and blastocyst stages were analyzed by nanoliquid chromatography coupled with tandem MS (nanoLC-MS/MS). A total of 2757 proteins were identified, of which 1950 were quantitatively analyzed. Principal component analysis of data showed a separation of embryo pools according to their developmental stage. Differentially abundant proteins (DAPs) between stages were clustered into 626 upregulated and 400 downregulated proteins with most significant changes at the time of EGA and blastocyst formation. The main pathways and processes overrepresented among upregulated proteins were RNA metabolism, protein translation and ribosome biogenesis whereas Golgi vesicle transport and protein processing in endoplasmic reticulum were overrepresented among downregulated proteins. This is the first comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo. These data provide a number of functional protein candidates and will be useful to evaluate the impact of in vitro conditions on embryo quality. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_05:40:30.914.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD030994 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | E Com |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-16 13:11:01 | ID requested | |
⏵ 1 | 2022-05-20 05:40:31 | announced | |
Publication List
Banliat C, Mah, é C, Lavigne R, Com E, Pineau C, Labas V, Guyonnet B, Mermillod P, Saint-Dizier M, . Front Cell Dev Biol, 10():863700(2022) [pubmed] |
Keyword List
submitter keyword: Embryo, proteomics, mass spectrometry, morula, blastocyst, cattle, development. |
Contact List
Charles Pineau |
contact affiliation | UMS Biosit - CNRS 3480 - Inserm 018 Campus de Beaulieu - Bâtiment. 24 - CS2407 263 Avenue du Général Leclerc 35042 RENNES CEDEX FRANCE |
contact email | proteome@univ-rennes1.fr |
lab head | |
E Com |
contact affiliation | Protim, Irset, Inserm U1085 |
contact email | emmanuelle.com@univ-rennes1.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030994
- Label: PRIDE project
- Name: Dynamic changes in the proteome of early bovine embryos developed in vivo