PXD030991

PXD030991 is an original dataset announced via ProteomeXchange.

Title	CK2β is a gatekeeper of focal adhesions regulating cell spreading
Description	CK2 is an hetero-tetrameric protein made of two CK2α catalytic subunits and two regulatory CK2β subunits. The free CK2α subunit and the tetrameric holoenzyme have distinct substrate specificity profiles suggesting that the spatiotemporal organization of the individual CK2 subunits observed in living cells is crucial in the control of the many cellular processes that are governed by this pleiotropic kinase. Indeed, sub-stoichiometric expression of CK2β compared to CK2α in a subset of breast cancer tumors was correlated with the induction of EMT markers and increased epithelial cell plasticity in breast carcinoma progression. Using phosphotyrosine profiling with affinity capture and LC/MS-MS, we show that decreased expression of CK2β in MCF10A mammary epithelial cells triggers the phosphorylation of a number of tyrosine phosphorylated proteins and promotes the striking activation of the FAK1-Src-PAX1 signaling pathway. Moreover, morphometric analyses also revealed that CK2β loss increases the number and the spatial distribution of focal adhesion signaling complexes. Together, our finding allows positioning CK2β as a gatekeeper for cell spreading by restraining the focal adhesion maturation and invasion of mammary epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:07.158.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030991
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hesse Anne-Marie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-16 09:30:35	ID requested
1	2022-07-05 02:14:59	announced
⏵ 2	2023-11-14 08:55:07	announced	2023-11-14: Updated project metadata.

10.6019/PXD030991;

submitter keyword: proteomics, Tyrosine phosphorylated proteins,CK2β depletion, LC-MS/MS

Yohann Couté
contact affiliation	Univ. Grenoble Alpes, INSERM, CEA, UMR BioSanté U1292, CNRS, CEA, FR2048, 38000 Grenoble, France
contact email	yohann.coute@cea.fr
lab head
Hesse Anne-Marie
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	anne-marie.hesse@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD030991

PRIDE project URI

• PRIDE
  1. PXD030991
     1. Label: PRIDE project
     2. Name: CK2β is a gatekeeper of focal adhesions regulating cell spreading