PXD030982

PXD030982 is an original dataset announced via ProteomeXchange.

Title	REGULATION OF THE RIBOSOME BIOGENESIS FACTOR HYVH1 BY SRC-MEDIATED PHOSPHORYLATION
Description	In this study, we investigate cellular effects of a novel Src-mediated phosphorylation site at Tyr179 on hYVH1. We observed that this phosphorylation event attenuates hYVH1's stress granule disassembly function, enhances shuttling of hYVH1 to the nucleus, and promotes hYVH1 partitioning to the 60S ribosomal subunit. Quantitative proteomics reveal that Src co-expression with hYVH1 reduces formation of ribosomal species that represent stalled intermediates through the alteration of associating factors that promote translational repression. Collectively, these results implicate hYVH1 as a novel Src substrate and is the first demonstrated role of tyrosine phosphorylation regulating the activity of a YVH1 ortholog. Moreover, the ribosome proteome alterations point to a collaborative function of hYVH1 and Src in maintaining translational fitness.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:33.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Panayiotis Vacratsis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-15 03:35:01	ID requested
1	2023-03-10 22:30:45	announced
⏵ 2	2023-11-14 08:37:37	announced	2023-11-14: Updated project metadata.

DaDalt AA, Bonham CA, Lotze GP, Luiso AA, Vacratsis PO, Src-mediated phosphorylation of the ribosome biogenesis factor hYVH1 affects its localization, promoting partitioning to the 60S ribosomal subunit. J Biol Chem, 298(12):102679(2022) [pubmed]

submitter keyword: Human, hYVH1, HeLa cells, HDMSe, ribosome profiling

Panayiotis Orestes Vacratsis
contact affiliation	Department of Chemistry and Biochemistry University of Windsor Canada
contact email	vacratsi@uwindsor.ca
lab head
Panayiotis Vacratsis
contact affiliation	University of Windsor
contact email	vacratsi@uwindsor.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD030982

PRIDE project URI

• PRIDE
  1. PXD030982
     1. Label: PRIDE project
     2. Name: REGULATION OF THE RIBOSOME BIOGENESIS FACTOR HYVH1 BY SRC-MEDIATED PHOSPHORYLATION