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PXD030971

PXD030971 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStaphylococcus aureus isolates from the gut and the blood are ‘separated’ by host barrier integrity
DescriptionThe opportunistic pathogen Staphylococcus aureus is carried asymptomatically by about one-third of the human population. Body sites known to be colonized by S. aureus are the skin, nasopharynx and gut. In particular, the mechanisms that allow S. aureus to pass the gut epithelial barrier and to invade the bloodstream are poorly understood. Therefore, our present study was aimed at investigating possible differences between gut-colonizing and bacteremia isolates of S. aureus. To this end, 74 gut-colonizing isolates from healthy individuals and 144 blood-culture isolates were characterized by whole-genome sequencing. Subsequently, the cellular and extracellular proteomes of six representative isolates were examined by mass spectrometry. Lastly, the virulence potential of these isolates was evaluated using infection models based on human gut epithelial cells, blood cells, and a small animal infection model. Intriguingly, our results show that gut-colonizing and bacteremia isolates with the same sequence type (ST1 or ST5) are very similar at the genomic and proteomic levels. Nonetheless, they display differences in virulence, but gut-colonizing isolates may be more virulent than bacteremia isolates and vice versa. Importantly, we show that the main decisive factor preventing infection of gut epithelial cells in vitro is the presence of a tight barrier. Based on our present observations, we propose that the integrity of the gut epithelial layer, rather than the pathogenic potential of a gut-colonizing S. aureus strain, is the main decisive factor that determines whether this colonizer will become an invasive pathogen.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:40:14.028.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSandra Maass
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListacetylated residue; monohydroxylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-14 08:28:43ID requested
12023-03-11 01:53:54announced
22023-11-14 08:40:16announced2023-11-14: Updated project metadata.
Publication List
Raineri EJM, Maa, ß S, Wang M, Brushett S, Palma Medina LM, Sampol Escandell N, Altulea D, Raangs E, de Jong A, Vera Murguia E, Feil EJ, Friedrich AW, Buist G, Becher D, Garc, í, a-Cobos S, Couto N, van Dijl JM, Staphylococcus aureus populations from the gut and the blood are not distinguished by virulence traits-a critical role of host barrier integrity. Microbiome, 10(1):239(2022) [pubmed]
Keyword List
submitter keyword: S. aureus
gut colonization
bacteremia
WGS
proteome
barrier
Contact List
Dörte Becher
contact affiliationDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, Greifswald, Germany
contact emaildbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliationUniversity of Greifswald, Department for Microbial Proteomics
contact emailsandra.maass@uni-greifswald.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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