PXD030959

PXD030959 is an original dataset announced via ProteomeXchange.

Title	Differential cell survival outcomes in response to diverse amino acid stress
Description	Mammalian cells respond to amino acid limitation by activating a coordinated response to preserve cell function and resources. Here we dissected amino acid stress sensing and adaptation and examined how prolonged arginine or leucine starvation alters cellular behavior of murine embryonic stem cells, that served as a model rapidly proliferating, non-transformed system. We used a temporal approach that combined proteomics, phosphoproteomics (EasyPhos), ubiqitinomics (TUBE-MS), and interaction proteomics (AP-MS) to provide a systematic investigation of the nutrient stress response and regulation networks of mTORC1 (with Castor1 and Sesn2 sensors) as well as cellular adaptation mechanisms.
HostingRepository	PRIDE
AnnounceDate	2025-04-01
AnnouncementXML	Submission_2025-04-01_04:16:19.801.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Steigenberger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-14 03:49:05	ID requested
⏵ 1	2025-04-01 04:16:20	announced

Dataset with its publication pending

submitter keyword: post-translational modification,proteomics, stress, phosphoproteomics, TUBE-MS, AP-MS, ubiquitinomics, Castor1, phosphorylation, protein-protein interaction, amino acid starvation, Sesn2, mTOR

Peter J.
contact affiliation	Department of Immunoregulation, Max Planck Institute of Biochemistry, 82152, Martinsried, Germany
contact email	murray@biochem.mpg.de
lab head
Barbara Steigenberger
contact affiliation	MPI of Biochemistry
contact email	steigenberger@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD030959

PRIDE project URI

• PRIDE
  1. PXD030959
     1. Label: PRIDE project
     2. Name: Differential cell survival outcomes in response to diverse amino acid stress