PXD030958

PXD030958 is an original dataset announced via ProteomeXchange.

Title	Electroneutral polymer nanodiscs enable interference-free probing of membrane proteins in a lipid-bilayer environment
Description	Membrane proteins can be examined in near-native lipid-bilayer environments with the advent of polymer-encapsulated nanodiscs. These nanodiscs self-assemble directly from cellular membranes, allowing in vitro probing of membrane proteins with techniques that had previously been restricted to soluble or detergent-solubilized proteins. Nonetheless, the high charge densities of existing polymers obstruct bioanalytical and preparative techniques through unspecific interactions. Here we describe a simple strategy for producing water-soluble, electroneutral polymer nanodiscs. Through attachment of a sulfobetaine group, the commercial polymers DIBMA and SMA can be easily converted into the charge-neutral maleimide derivatives, sulf DIBMA and sulf SMA, which readily extract proteins and phospholipids from artificial and cellular membranes to form nanodiscs. Crucially, the electroneutrality of the new nanodiscs averts unspecific interactions, thereby enabling reliable lab-on-a-chip detection of protein/lipid interactions and in vitro translation of membrane proteins. Finally, we create a library containing thousands of human membrane proteins and use proteome profiling by mass spectrometry to show the preservation of protein complexes in electroneutral nanodiscs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:05.523.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-14 03:48:25	ID requested
1	2023-01-17 00:38:46	announced
⏵ 2	2023-11-14 08:55:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: membrane proteins,DIBMA nanodiscs

Markus Räschle
contact affiliation	Molecular Genetics, Technical University Kaiserslautern
contact email	raeschle@bio.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD030958

PRIDE project URI

• PRIDE
  1. PXD030958
     1. Label: PRIDE project
     2. Name: Electroneutral polymer nanodiscs enable interference-free probing of membrane proteins in a lipid-bilayer environment