PXD030951

PXD030951 is an original dataset announced via ProteomeXchange.

Title	Plasma Proteomic Changes of Atherosclerosis After Exercise in ApoE knockout Mice
Description	Atherosclerosis is the preliminary cause of coronary artery disease, one of the diseases that ac-count for the largest number of fatal mortalities. Physical activity is an effective strategy to restrain atherosclerosis from deterioration. Evidence indicated that the changes of proteomic profile is highly associated with the atherosclerosis development, but the mechanism behind the exercise for atherosclerosis amelioration has not yet been investigated from the proteomics perspective. Hence, the proteomic profiles could further elucidate the systematic effects of exercise interven-tion on ApoE knockout atherosclerotic model and high fat diet intervention. In current study, Apoeem1Narl/Narl mice were randomly allocated into a normal diet (ND), western diet (WD) and WD with 12 weeks exercise intervention (WD EX) groups. The plasm proteome between WD and WD EX demonstrate the significant difference, and ten major pathways, including cardiovascular disease (CVD)–hematological disease, cellular compromise–inflammatory response, protein synthesis, connective tissue disorders, cellular movement–immune cell tracking, inflammatory response, etc., were generated by the IPA analysis. The fourteen proteins (PROS, PROZ, C2, F5, C5, SERPINA 10, FGB, FGG, CFB, F12, CRP, CFHR1, HABP2, and PPIA) critically involved in CVD–hematological disease pathway showed significant difference among groups. The PROS, F5, C5, FGG, and CFB levels associated with thrombosis and atherosclerosis induced by WD were significantly decreased by exercise intervention in WD EX. Furthermore, the F5 and FGG were well-demonstrated the important roles for thrombosis of atherosclerotic pathogenesis but the complement factor C5 in the development of atherosclerosis is not fully understood. In current study, the exercise could significantly alleviate the significantly elevated C5 and inflammation induced by WD group in accordance to amelioration of atherosclerosis. Therefore, exercise could mitigate chemotaxis through the modulation of the C5 level and innate immunity, thereby alle-viating the pathogenesis of atherosclerosis in western-diet induced obese mice.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_09:13:44.855.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030951
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chen-Chung Liao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-14 00:41:48	ID requested
⏵ 1	2022-05-25 09:13:45	announced

Liao CC, Xu JW, Huang WC, Chang HC, Tung YT, Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice. Biology (Basel), 11(2):(2022) [pubmed]

submitter keyword: atherosclerosis

complement factor C5

exercise

proteomic changes

chemotaxis

Chen-Chung Liao
contact affiliation	Metabolomics-Proteomics Research Center, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan
contact email	ccliao@nycu.edu.tw
lab head
Chen-Chung Liao
contact affiliation	Proteomics Research Center,National Yang-Ming University
contact email	ccliao@ym.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030951
     1. Label: PRIDE project
     2. Name: Plasma Proteomic Changes of Atherosclerosis After Exercise in ApoE knockout Mice