PXD030937

PXD030937 is an original dataset announced via ProteomeXchange.

Title	The Role of Acetylated CBP on MARK2 activity
Description	This study investigates the interactions between two enzymes involved in regulating the protein tau’s PTMs: the kinase MARK2 and the acetyltransferase CBP. Western blot analysis revealed that CBP-mediated acetylation was increased in the absence of MARK2, indicating a possible negative feedback loop, where MARK2 is inhibiting CBP acetylation. In contrast, inactive MARK2 (MARK2-KR) was strongly acetylated in the presence of acetyl-CBP, consistent with the preferential CBP binding and targeting of the inactive MARK2 conformation. To determine the specific lysine residues in MARK2 that are subject to acetylation in the presence of CBP, we immunopurified MARK2-KR alone (as a control) and CBP-acetylated MARK2-KR. We analyzed the immunopurified MARK2-KR by mass spectrometry-based proteomics to determine differential acetyl and phosphorylation sites.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_07:24:47.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Herring
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-13 04:12:04	ID requested
⏵ 1	2022-06-09 07:24:48	announced

Tabassum Z, Tseng JH, Isemann C, Tian X, Chen Y, Herring LE, Cohen TJ, Identification of a reciprocal negative feedback loop between tau-modifying proteins MARK2 kinase and CBP acetyltransferase. J Biol Chem, 298(6):101977(2022) [pubmed]

submitter keyword: phosphorylation, MARK2

Todd Cohen
contact affiliation	Associate Professor, Department of Neurology, UNC-Chapel Hill
contact email	toddcohen@neurology.unc.edu
lab head
Laura Herring
contact affiliation	UNC-Chapel Hill
contact email	laura_herring@med.unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030937
     1. Label: PRIDE project
     2. Name: The Role of Acetylated CBP on MARK2 activity