PXD030929

PXD030929 is an original dataset announced via ProteomeXchange.

Title	Heterologously secreted MbxA from Moraxella bovis induces a membrane blebbing response of the host cell
Description	Many proteins of the Repeats in Toxins (RTX) protein family are toxins of Gram-negative pathogens including hemolysin A (HlyA) of uropathogenic E. coli. RTX proteins are secreted via Type I secretion systems (T1SS) and adopt their native conformation in the Ca2+-rich extracellular environment. Here we employed the E. coli HlyA T1SS as a heterologous system for the RTX toxin MbxA from the bovine pathogen Moraxella bovis. In E. coli the HlyA system successfully activates the heterologous MbxA substrate by acylation and secretes the precursor proMbxA and active MbxA allowing purification of both species. The activating E. coli acyltransferase HlyC recognizes the acylation sites in MbxA, but unexpectedly in a different acylation pattern as for its endogenous substrate HlyA.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:39:54.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Moraxella bovis; NCBI TaxID: 476;
ModificationList	monohydroxylated residue; N-acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-13 01:48:13	ID requested
1	2023-03-11 01:11:34	announced
⏵ 2	2023-11-14 08:39:58	announced	2023-11-14: Updated project metadata.

Erenburg IN, H, ä, nsch S, Chacko FM, Hamacher A, Wintgens S, Stuhldreier F, Poschmann G, Spitz O, St, ü, hler K, Wesselborg S, Hegemann JH, Smits SHJ, Weidtkamp-Peters S, Schmitt L, Heterologously secreted MbxA from Moraxella bovis induces a membrane blebbing response of the human host cell. Sci Rep, 12(1):17825(2022) [pubmed]

submitter keyword: secretion, membrane blebbing,RTX toxins, acylation, cell membrane damage

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Institute of Molecular Medicine I Heinrich Heine University Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030929
     1. Label: PRIDE project
     2. Name: Heterologously secreted MbxA from Moraxella bovis induces a membrane blebbing response of the host cell