PXD030920

PXD030920 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of drug trichosanthin addition to BeWo cell line
Description	Trichosanthin (TCS) is a traditional Chinese herbal medicine to treat some gynecological diseases. Its effective component has diverse biological function, including antineoplastic activity. The human trophoblast cell line BeWo was chosen as an experimental model for in vitro test of drug screen for anti-cancer property of TCS. MTT method was used in this study to get primary screen result. The result showed that 100 mM had the best IC50 value. Proteomics analysis was then performed for further investigation for the drug effect of TCS to the BeWo cell line. In this differential proteomic expression analysis, total protein extract from BeWo cell line and their protein expression level after the drug treatment were compared by 2DE. Then 24 unique three-fold differential expressed proteins (DEPs) were successfully identified by MALDI-TOF-TOF-MS. Label-free proteomics as complemental method was run for the same experimental procedure. There are two proteins were identified in both 2DE and label free methods. Among those identified proteins, bioinformatics analysis shows the importance of pathway and signal transduction and gives us the potential possibility for the disease treatment hypothesis.
HostingRepository	iProX
AnnounceDate	2022-01-13
AnnouncementXML	Submission_2022-10-10_00:00:45.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-13 00:00:20	ID requested
1	2022-01-13 00:00:33	announced
⏵ 2	2022-10-10 00:00:46	announced	2022-10-10: Update publication information.

Hu Y, Yao J, Wang Z, Liang H, Li C, Zhou X, Yang F, Zhang Y, Jin H, Comparative Proteomic Analysis of Drug Trichosanthin Addition to BeWo Cell Line. Molecules, 27(5):(2022) [pubmed]

submitter keyword: BeWo Proteome

Yang Zhang
contact affiliation	Institutes of Biomedical Sciences, Fudan University
contact email	zhangyang@fudan.edu.cn
lab head
Yang Zhang
contact affiliation	Institutes of Biomedical Sciences, Fudan University
contact email	zhangyang@fudan.edu.cn
dataset submitter

iProX dataset URI