PXD030902

PXD030902 is an original dataset announced via ProteomeXchange.

Title	1A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transporters
Description	Cells rapidly remodel their proteomes to align their cellular metabolism to environmentalconditions. Ubiquitin E3 ligases enablethis response, by facilitatingrapid andreversible changes to protein stability, localization, or interaction partners. In S. cerevisiae, the GID E3 ligase regulatesthe switch from gluconeogenic to glycolytic conditions throughinduction and incorporation of the substrate receptorsubunitGid4, whichpromotes the degradation of gluconeogenic enzymes. Here,we show an alternative substrate receptor, Gid10, which is induced in response to changes in temperature, osmolarity and nutrient availability,andregulates the ART-Rsp5 pathway. Art2 levels are elevated upon GID10deletion, a crystal structure shows the basis for Gid10-Art2 interactions, andGid10 directs a GID E3 ligase complex to ubiquitinate Art2. We also findthat the GID E3 ligase affects the flux of plasma membrane nutrient transportersduring heat stress. The data revealGID as a system of E3 ligases with metabolic regulatory functions outside of glycolysis and gluconeogenesis, controlled by distinct stress-specific substrate receptors.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_03:09:27.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-12 00:23:29	ID requested
⏵ 1	2022-03-02 03:09:27	announced

Dataset with its publication pending

submitter keyword: ubiquitin E3 ligase, nutrient signaling, metabolism, GID, Rsp5, proteomics, DIA, MS

Matthias Mann
contact affiliation	Head of the Department of Proteomics and Signal Transduction at the MPI Biochemistry
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD030902

PRIDE project URI

• PRIDE
  1. PXD030902
     1. Label: PRIDE project
     2. Name: 1A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transporters