PXD030901

PXD030901 is an original dataset announced via ProteomeXchange.

Title	Proteins are well-preserved in shells toasted at 300°C revealed by proteomics
Description	The development of protein anti-degradation strategy is important for their storage at ambient conditions. Despite that it is known that biominerals, typical inorganic-organic composites, can preserve proteins at room temperature for a long time, it is unclear the extent of protein degradation under high temperatures. In this study, we examined protein remaining in the toasted abalone shell under high temperatures (200 and 300 °C) by biomineral proteomics method. Surprisingly, 24 proteins including carbonic anhydrase, hemocyanin, actin can still be identified from shells even after toasting under 300°C, not much decreased compared to that in the 200°C-treated and the native shell. However, the microstructure and composition (both mineral and organic matrix) of shells were altered significantly revealed by scanning electron microscopy, infrared spectroscopy, and X-ray diffraction. The well-preserved of proteins may be partially due to the sacrifice of mineral/organic interfaces and the formation of nanopores in the shell at high temperatures. Moreover, the extracted proteins from both groups were able to affect calcium carbonate in vitro. This study has potential implications in various fields such as protein storage at high temperatures and palaeoproteomics.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:31:34.922.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuang Liu
SpeciesList	scientific name: Haliotis discus hannai; NCBI TaxID: 42344;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-12 00:21:51	ID requested
1	2022-06-30 20:19:39	announced
⏵ 2	2023-11-14 08:31:35	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: shell matrix proteins

biomineralization

high temperature

Chuang Liu
contact affiliation	Hohai University, Nanjing, China
contact email	helloliuchuang@163.com
lab head
Chuang Liu
contact affiliation	Hohai University, Nanjing, China
contact email	helloliuchuang@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD030901

PRIDE project URI

• PRIDE
  1. PXD030901
     1. Label: PRIDE project
     2. Name: Proteins are well-preserved in shells toasted at 300°C revealed by proteomics